Reference "Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the alpha-subunit of GTP-binding protein"

Reference ID

6445

Title

Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the alpha-subunit of GTP-binding protein

Source

Proceedings of the National Academy of Sciences of the United States of America, 1999, vol. 96, pp. 10284-10289

Authors (5)

Ashikari-M	Wu-J
Yano-M	Sasaki-T
Yoshimura-A

Abstract

A rice Dwarf 1 gene was identified by using a map-based cloning strategy. Its
recessive mutant allele confers a dwarf phenotype. Linkage analysis revealed
that a cDNA encoding the alpha-subunit of GTP- binding protein cosegregated with
d1 in 3,185 d1 segregants. Southern hybridization analysis with this cDNA as a
probe showed different band patterns in several d1 mutant lines. In at least
four independent d1 mutants, no gene transcript was observed by Northern
hybridization analysis. Sequencing analysis revealed that an 833-bp deletion had
occurred in one of the mutant alleles, which resulted in an inability to express
GTP-binding protein. A transgenic d1 mutant with GTP-binding protein gene
restored the normal phenotype. We conclude that the rice Dwarf 1 gene encodes
GTP-binding protein and that the protein plays an important role in plant growth
and development. Because the d1 mutant is classified as gibberellin-insensitive,
we suggest that the GTP- binding protein might be associated with gibberellin
signal transduction.