Reference "Biotin synthesis in higher plants: purification and characterization of bioB gene product equivalent from Arabidopsis thaliana overexpressed in Escherichia coli and its subcellular localization in pea leaf cells"

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E.g., Wessler, regeneration, PubMed ID 17578919.

Reference "Biotin synthesis in higher plants: purification and characterization of bioB gene product equivalent from Arabidopsis thaliana overexpressed in Escherichia coli and its subcellular localization in pea leaf cells"

Reference ID

54996

Title

Biotin synthesis in higher plants: purification and characterization of bioB gene product equivalent from Arabidopsis thaliana overexpressed in Escherichia coli and its subcellular localization in pea leaf cells

Source

FEBS Lett, 1997, vol. 419, pp. 206-210

Authors (3)

Baldet-P	Alban-C
Douce-R

Abstract

Biotin synthase catalyses the final step in the biotin biosynthetic pathway and
is encoded by the bioB gene in Escherichia coli. To investigate the conversion
of dethiobiotin to biotin in the plant kingdom, the cDNA encoding the bioB gene
product equivalent from Arabidopsis thaliana was used to construct an E. coli
overexpression strain. The purified A. thaliana bioB gene product is a homodimer
(100 kDa) with a reddish color and has an absorbance spectrum characteristic of
protein with [2Fe-2S] clusters. Its intracellular compartmentation in pea leaves
discloses a unique polypeptide of 39 kDa within the matrix of mitochondria.

Database Cross-References (1)

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