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E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation"
Reference ID 54962
Title Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation
Source Nature, 2013, vol. 496, pp. 91-95
Authors (50)
Abstract About 8,000 years ago in the Fertile Crescent, a spontaneous hybridization of
the wild diploid grass Aegilops tauschii (2n = 14; DD) with the cultivated
tetraploid wheat Triticum turgidum (2n = 4x = 28; AABB) resulted in hexaploid
wheat (T. aestivum; 2n = 6x = 42; AABBDD). Wheat has since become a primary
staple crop worldwide as a result of its enhanced adaptability to a wide range
of climates and improved grain quality for the production of baker's flour. Here
we describe sequencing the Ae. tauschii genome and obtaining a roughly 90-fold
depth of short reads from libraries with various insert sizes, to gain a better
understanding of this genetically complex plant. The assembled scaffolds
represented 83.4% of the genome, of which 65.9% comprised transposable elements.
We generated comprehensive RNA-Seq data and used it to identify 43,150 protein-
coding genes, of which 30,697 (71.1%) were uniquely anchored to chromosomes with
an integrated high-density genetic map. Whole-genome analysis revealed gene
family expansion in Ae. tauschii of agronomically relevant gene families that
were associated with disease resistance, abiotic stress tolerance and grain
quality. This draft genome sequence provides insight into the environmental
adaptation of bread wheat and can aid in defining the large and complicated
genomes of wheat species.

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