Reference "Sequencing and De Novo Transcriptome Assembly of Brachypodium sylvaticum (Poaceae)"

Reference ID

54957

Title

Sequencing and De Novo Transcriptome Assembly of Brachypodium sylvaticum (Poaceae)

Source

Applications in Plant Sciences, 2013, vol. 1, pp. -

Authors (8)

Fox-S	Preece-J
Kimbrel-J-A	Marchini-G-L
Sage-A	Youens-Clark-K
Cruzan-M	Jaiswal-P

Abstract

Brachypodium sylvaticum (Huds.) P. Beauv. (slender falsebrome; Poaceae), with an
estimated genome size of 470 Mb and 17 chromosomes (Foote et al., 2004), is a
perennial bunchgrass native to Europe, Asia, and North Africa and is closely
related to the bioenergy feedstock model grass B. distachyon (L.) P. Beauv.
(Wolny et al., 2011), which has a sequenced genome of 272 Mb and five
chromosomes. In its native range, B. sylvaticum occurs in habitats ranging from
forest understory to open meadows and tolerates conditions from full shade to
full sun (Holten, 1980; Long, 1989; Aarrestad, 2000; Kirby and Thomas, 2000). In
the United States, B. sylvaticum is invasive and listed as a noxious weed
covering the west coast of California, Oregon, and Washington (Oregon Department
of Agriculture, 2009; Washington State Department of Agriculture, 2009; Lionakis
Meyer and Effenberger, 2010). It is also expanding into the eastern United
States where it has been reported in Missouri and Virginia (Roy, 2010). In
Oregon, B. sylvaticum forms thick monocultures in open forests at elevations
from nearly sea level to approximately 1200 m. It threatens the endangered
Oregon Willamette Valley oak savanna ecosystem by replacing the native flora and
reduces habitat for rare butterflies (Kaye and Blakeley-Smith, 2006; Severns and
Warren, 2008). False brome is shade tolerant (Murchie and Horton, 1997; Holmes
et al., 2010), which makes it a particularly dangerous invasive threat to
undisturbed habitats (Martin et al., 2009).