Reference "Identification and characterization of cDNA clones encoding plant calreticulin in barley"

Reference ID

5367

Title

Identification and characterization of cDNA clones encoding plant calreticulin in barley

Source

The Plant cell, 1994, vol. 6, pp. 835-843

Authors (4)

Chen-F	Hayes-P-M
Mulrooney-D-M	Pan-A

Abstract

Two cDNA clones (CRH1 and CRH2) homologous to animal calreticulin, a major
calcium storage protein in the lumen of the endoplasmic reticulum, were isolated
from an ovary cDNA library of barley through differential screening. The two
clones differ in the 3' untranslated region and the 5' region that encodes a
putative N-terminal signal sequence. CRH1 was mapped to the minus arm of
chromosome 1. CRH2 was mapped to the minus arm of chromosome 2. The deduced
amino acid sequences share 50 to 55% identity with animal calreticulins and
exhibit the same three-zone characteristic. Recombinant protein stained blue
with Stains-all and bound 45Ca2+ when transferred to nitrocellulose membranes. A
native protein of approximately 55 kD was identified in ovary extract. Elevated
gene expression was observed in ovaries 1 day after pollination and during early
embryogenesis. CRH1 was expressed at a higher level than CRH2. These studies
demonstrate the presence of calreticulin in plant cells and its developmental
regulation in fertilization.