Reference "A modified chemical procedure for rapid determination of glucosamine and its application for estimation of mold growth in peanut kernels and koji"

Reference ID

531

Title

A modified chemical procedure for rapid determination of glucosamine and its application for estimation of mold growth in peanut kernels and koji

Source

Journal of agricultural and food chemistry, 1999, vol. 47, pp. 1999-2004

Authors (2)

Chen-W

Chiou-R-Y

Abstract

One-step hydrolysis of chitin to release glucosamine for quantitation was
achieved by combining a chitin-containing sample (10-200 mg of sample size) in a
test tube with 1 mL of 10 M HCl followed by vacuum treatment for 10 min,
incubation at 28 degrees C for 30 min, replenishment with 3 mL of deionized
water, nitrogen flushing, screw capping, and heat treatment at 140 degrees C for
60 min. A phosphate buffer solution (pH 12.5, 0.2 M) was effective in pH
stabilization and enhancing colorimetric determination of glucosamine content.
When the modified procedure was applied to analyze glucosamine content in the
mycelia of various molds, glucosamine content varied mainly depending on mold
species. In estimations of mold growth of the uninoculated peanut kernels
incubated under a humidified condition for 5 weeks, cooked rice and soybean
inoculated with conidia of Aspergillus oryzae for koji preparation, logarithms
of the internal mold populations and glucosamine contents both increased with
increases of incubation time. The modified procedure provided a rapid and
reliable estimation of mold growth in various substrates.