Reference "A 45-bp proximal region containing AACA and GCN4 motif is sufficient to confer endosperm-specific expression of the rice storage protein glutelin gene, GluA-3"

Reference ID

5180

Title

A 45-bp proximal region containing AACA and GCN4 motif is sufficient to confer endosperm-specific expression of the rice storage protein glutelin gene, GluA-3

Source

FEBS letters, 1996, vol. 383, pp. 213-218

Authors (3)

Yoshihara-T	Washida-H
Takaiwa-F

Abstract

A 45-bp proximal region of the rice glutelin promoter (-104/-60) containing two
putative cis-elements, the AACA motif and GCN4 motifs, was fused to a truncated
CaMV 35S promoter (-90/+9; -90 delta 35S)/GUS. The 45-bp fragment specifically
enhanced the promoter activity in endosperm tissue of transformed tobacco. A
substitution mutation of the GCN4 motif reduced the promoter activity, whereas
mutation of the AACA motif increased the activity in the embryo as well as in
the endosperm. These results suggest that the GCN4 motif generally enhances the
promoter activity but that the combination of the two motifs confers the
endosperm specificity. Furthermore, the function of the two motifs was dependent
on the orientation and/or distance from a G-box element in -90 delta 35S,
suggesting that synergistic interaction between the factors that recognize those
motifs and the G-box element is important for transcriptional regulation.