Reference "cis-regulatory elements responsible for quantitative regulation of the rice seed storage protein glutelin GluA-3 gene"

Reference ID

5179

Title

cis-regulatory elements responsible for quantitative regulation of the rice seed storage protein glutelin GluA-3 gene

Source

Plant & cell physiology, 1996, vol. 37, pp. 107-111

Authors (2)

Yoshihara-T

Takaiwa-F

Abstract

The 897 bp 5' flanking region of the rice storage protein glutelin gene (GluA-3)
was analyzed to define cis-regulatory elements responsible for the seed specific
expression by progressive 5' deletions and gain-of- function experiments using a
truncated CaMV 35S promoter (-90/+9). These constructs were transcriptionally
fused either to the firefly luciferase (LUX) or beta-glucuronidase (GUS)
reporter gene and introduced into tobacco. It was shown that a 121 bp sequence
between - 437 and -317 from the transcriptional start site is important for the
quantitative regulation of the GluA-3 gene expression. This region contained at
least two functionally different cis-regulatory elements. One acts as a general
positive element and the other is involved in a specific negative regulation in
non-seed tissues.