Reference "Rice alcohol dehydrogenase genes: anaerobic induction, organ specific expression and characterization of cDNA clones"

Reference ID

5008

Title

Rice alcohol dehydrogenase genes: anaerobic induction, organ specific expression and characterization of cDNA clones

Source

Plant molecular biology, 1989, vol. 13, pp. 53-68

Authors (2)

Xie-Y

Wu-R

Abstract

Anaerobiosis rapidly induces alcohol dehydrogenase (ADH), an enzyme of the
fermentation pathway, in different parts of rice seedlings. After initiation of
anaerobiosis, the activity of the enzyme increases linearly for 3 days or more.
The ADH activity is anaerobically inducible even in mature rice leaves in
contrast to maize which shows no induction in mature leaves. Rice ADH activity
can also be induced by an auxin analog, 2,4-dichlorophenoxyacetic acid, under
aerobic conditions. The experimental results show that anaerobiosis increases
the ADH mRNA level, indicating that the ADH enzyme is regulated at the
transcriptional level. Starch gel electrophoresis of a protein extract from rice
shows 3 distinct forms of ADH. The amounts of the 3 forms vary with the organ,
suggesting that the expression of ADH genes is organ-specific. Sequencing data
show that the two different cloned cDNA copies of ADH mRNAs are derived from two
different genes.