Reference "Genetic and physical mapping of telomeres and microsatellites of rice"

Reference ID

4976

Title

Genetic and physical mapping of telomeres and microsatellites of rice

Source

Plant molecular biology, 1993, vol. 22, pp. 861-872

Authors (2)

Wu-K-S

Tanksley-S-D

Abstract

Telomeres and telomere-associated satellites of rice were genetically and
physically analyzed by pulsed-field gel electrophoresis (PFGE) using Arabidopsis
telomeric DNA and rice satellite sequences as probes. We demonstrate that
Arabidopsis telomeric sequences hybridize to rice telomeres under the conditions
of high stringency. Using the Arabidopsis probe, multiple, discrete telomeric
fragments could be identified on pulsed-field gel blots of rice DNAs digested
with rare- cutting restriction enzymes. Most of the telomeric bands larger than
300 kb are physically linked with satellite bands as revealed by PFGE. Some of
the telomeric and satellite bands segregate in a Mendelian fashion and are
highly reproducible. Three such telomeric bands have been mapped to the distal
ends of RFLP linkage groups: Telsm-1 on chromosome 8, Telsa-1 on chromosome 9
and Telsm-3 on chromosome 11. One segregating satellite band was mapped to an
internal region of chromosome 10. Telomeric fragments were shown not only to be
genetically linked to but also physically linked (based on PFGE) to the terminal
RFLP markers. The physical distance from telomeric sequences to a distal RFLP
marker, r45s gene, on chromosome 9, is 200 kb while the distance from telomeric
sequences to RG98, a terminal RFLP marker on chromosome 11, is 260 kb. Physical
maps of the telomere regions of chromosome 9 and chromosome 11 are presented.