Reference "Abundance, polymorphism and genetic mapping of microsatellites in rice"

Reference ID

4974

Title

Abundance, polymorphism and genetic mapping of microsatellites in rice

Source

Molecular & General Genetics, 1993, vol. 241, pp. 225-235

Authors (2)

Wu-K-S

Tanksley-S-D

Abstract

Dinucleotide microsatellites have been characterized and used as genetic markers
in rice. Screening of a rice genomic library with poly(dG-dA).(dC-dT) and poly(dG-dT).(dC-
dA) probes indicated that (GA)n repeats occurred, on average, once every 225 kb
and (GT)n repeats once every 480 kb. DNA sequencing of ten randomly selected
microsatellites indicated that the numbers of repeats ranged from 12 to 34 and
that the patterns of microsatellites in rice were similar to those of humans and
other mammals. Primers to these microsatellite loci as well as to four published
microsatellite-containing sequences have been designed and degrees of
polymorphism has been examined with 20 rice accessions. Multiple alleles,
ranging from 5 to 11, have been observed at all the microsatellite loci in 20
rice accessions. Alleles specific to two cultivated subspecies, indica and
japonica, were found in some microsatellite loci. Heterozygosity values of all
the microsatellite markers were significantly higher than those of RFLP markers,
based upon a parallel comparison. Ten microsatellite loci have been genetically
mapped to four rice chromosomes. The genomic distribution of microsatellites
appears to be random in rice. [References: 46]