Reference "A complete sequence of the rice sucrose synthase-1 (RSs1) gene"

Reference ID

4836

Title

A complete sequence of the rice sucrose synthase-1 (RSs1) gene

Source

Plant molecular biology, 1992, vol. 19, pp. 881-885

Authors (3)

Wang-M-B	Boulter-D
Gatehouse-J-A

Abstract

Using a fragment of the maize sucrose synthase gene Sh-1 as probe, the rice
genome was shown to contain at least three genes encoding sucrose synthase. One
of these genes was isolated from a genomic library, and its full sequence,
including 1.7 kb of 5' flanking sequence and 0.9 kb of 3' flanking sequence, is
reported. The new rice gene, designated RSs1, is highly homologous to maize Sh-1
(approx. 94% identity in derived amino acid sequence), and contains an identical
intron-exon structure (16 exons and 15 introns). Both RSs1 and maize Sh-1 show
similar sequence homologies to a second rice sucrose synthase gene described
recently (designated RSs2, Yu et al. (1992) Plant Mol Biol 18: 139-142),
although both the rice genes predict an extra 6 amino acids at the C-terminus of
the protein when compared to the maize gene. The RSs1 5' flanking sequence
contains a number of promoter-like sequences, including putative protein-binding
regions similar to maize zein genes.