Reference "Promoter elements required for sugar-repression of the RAmy3D gene for alpha-amylase in rice"

Reference ID

4579

Title

Promoter elements required for sugar-repression of the RAmy3D gene for alpha-amylase in rice

Source

FEBS letters, 1998, vol. 428, pp. 275-280

Authors (3)

Toyofuku-K	Umemura-T
Yamaguchi-J

Abstract

There is increasing evidence showing that cereal alpha-amylase gene expression
is controlled not only by the classical hormonal regulation, but also by feed-
back sugar repression. We demonstrated by in situ hybridization that the sugar
repression of rice alpha-amylase gene RAmy3D takes place in scutellar epithelium
cells of callus-forming rice embryos. We also used a transient expression system
to study the cis- acting elements involved in the sugar repression of the RAmy3D
promoter activity. Site-directed mutagenesis of the 50-bp nucleotide sequence
from -172 to -123 revealed that consensus sequences of G motif (TACGTA) and
TATCCA T/C motif (GATA motif as its antisense sequence) are responsible for
sugar repression. The promoter sequences required for sugar repression are
reported and discussed.