Reference "Structure of a rice beta-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors"

Reference ID

4127

Title

Structure of a rice beta-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors

Source

Plant molecular biology, 1992, vol. 18, pp. 33-45

Authors (4)

Simmons-C-R	Litts-J-C
Huang-N	Rodriguez-R-L

Abstract

A rice beta-glucanase gene was sequenced and its expression analyzed at the
level of mRNA accumulation. This gene (Gns1) is expressed at relatively low
levels in germinating seeds, shoots, leaves, panicles and callus, but it is
expressed at higher levels in roots. Expression in the roots appears to be
constitutive. Shoots express Gns1 at much higher levels when treated with
ethylene, cytokinin, salicylic acid, and fungal elicitors derived from the
pathogen Sclerotium oryzae or from the non-pathogen Saccharomyces cereviseae.
Shoots also express Gns1 at higher levels in response to wounding. Expression in
the shoots is not significantly affected by auxin, gibberellic acid or abscisic
acid. The beta-glucanase shows 82% amino acid similarity to the barley 1,3;1,4-beta-D-
glucanases, and from hybridization studies it is the beta-glucanase gene in the
rice genome closest to the barley 1,3;1,4- beta-glucanase EI gene. The mature
peptide has a calculated molecular mass of 32 kDa. The gene has a large 3145 bp
intron in the codon for the 25th amino acid of the signal peptide. The gene
exhibits a very strong codon bias of 99% G + C in the third position of the
codon in the mature peptide coding region, but only 61% G + C in the signal
peptide region.