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E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "Rapid isolation of a rice waxy sequence: a simple PCR method for the analysis of recombinant plasmids from intact Escherichia coli cells"
Reference ID 4065
Title Rapid isolation of a rice waxy sequence: a simple PCR method for the analysis of recombinant plasmids from intact Escherichia coli cells
Source Gene, 1991, vol. 98, pp. 243-248
Authors (2)
Abstract Polymerase chain reaction (PCR) is an efficient method for obtaining a desired
nt sequence if both required primers can hybridize to the target DNA molecule
specifically. A rapid and simple PCR-based method for analyzing plasmids using
intact cells was established. An attempt to target a rice waxy sequence by PCR
using homologous primers was also carried out. In three cases, specific
fragments were amplified and their nucleotide sequences were determined.
However, the cloned rice waxy gene contained base substitution mutations. The
cumulative frequency of mutation after 30 polymerization cycles was estimated to
be one in 500 bp.
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