Reference "Cloning of a cDNA that encodes farnesyl diphosphate synthase and the blue-light-induced expression of the corresponding gene in the leaves of rice plants"

Reference ID

3827

Title

Cloning of a cDNA that encodes farnesyl diphosphate synthase and the blue-light-induced expression of the corresponding gene in the leaves of rice plants

Source

Biochimica et biophysica acta, 1997, vol. 1350, pp. 240-246

Authors (5)

Sanmiya-K	Iwasaki-T
Matsuoka-M	Miyao-M
Yamamoto-N

Abstract

A cDNA encoding farnesyl diphosphate synthase (FPPS), a key enzyme in isoprenoid
biosynthesis, was isolated from a cDNA library constructed from mRNA that had
been prepared from etiolated rice (Oriza sativa L. variety Nipponbare) seedlings
after three hours of illumination by a subtraction method. The putative
polypeptide deduced from the 1289 bp nucleotide sequence consisted of 353 amino
acids and had a molecular mass of 40 676 Da. The predicted amino acid sequence
exhibited high homology to those of FPPS from Arabidopsis (73% to type 1, 72% to
type 2) and white lupin (74%). Southern blot analysis showed that the rice
genome might contain only one gene for FPPS. The highest level of expression of
the gene was demonstrated in leaves by RNA blot analysis. Moreover, light, in
particular blue light, effectively enhanced expression of the gene.