Reference "Rice gibberellin-insensitive gene homolog, OsGAI, encodes a nuclear- localized protein capable of gene activation at transcriptional level"

Reference ID

3231

Title

Rice gibberellin-insensitive gene homolog, OsGAI, encodes a nuclear- localized protein capable of gene activation at transcriptional level

Source

Gene, 2000, vol. 245, pp. 21-29

Authors (4)

Ogawa-M	Kusano-T
Katsumi-M	Sano-H

Abstract

This paper reports isolation and properties of a rice gene, OsGAI, a putative
homolog of the GAI of Arabidopsis thaliana. OsGAI encodes a polypeptide of 625
amino acids, which shows 53-55% identity to GAI and RGA from A. thaliana, and
85% identity to wheat rht-D1a and maize d8. Genomic DNA blot analysis indicated
the OsGAI to be a single-copy gene in the rice genome. RNA blot hybridization
showed that OsGAI transcripts increased within 6h upon GA(3) but not ABA
application. This GA-induced increment in OsGAI transcripts did not require de
novo protein synthesis. High levels of OsGAI transcripts were detected in nodes,
internodes, leaf sheaths and ears of adult plants and leaf sheaths of young
seedlings, where GA enhances cell elongation and division. Transiently expressed
OsGAI-GFP fusion protein located to the nucleus in onion epidermal cells.
Transactivation assays clearly indicated that OsGAI protein is a transcriptional
activator or a coactivator.