Reference "Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice"

Reference ID

3121

Title

Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice

Source

Genome ( National Research Council Canada ), 1996, vol. 39, pp. 26-30

Authors (2)

Naqvi-N-I

Chattoo-B-B

Abstract

Two randomly amplified polymorphic DNA (RAPD) markers, OPF6(2700) and
OPH18(2400), tightly linked to Pi-10, a dominant blast-resistance gene
conferring complete resistance to isolate 106 (international race IB46) of the
blast fungus were identified. To derive sequence characterized amplified regions
(SCARs) from OPF6(2700) and OPH18(2400), these amplified RAPD products were
cloned and sequenced. Nucleotide sequence information, obtained for each end of
the two linked RAPD markers, was used to design 24-mer oligonucleotide primers
for PCR amplification of the respective SCARs. Polymorphisms appearing as
differences in the length of the SCAR's alternate alleles were considered for
the indirect selection of Pi-10. Such polymorphisms converted the linked
dominant RAPD loci into codominant SCAR markers and also facilitated the
indirect scoring of the blast-resistant and blast-susceptible genotypes. The
development of length variant codominant SCAR markers linked to a major gene for
blast resistance in rice is described. The codominant SCARs will facilitate marker-
assisted selection of the Pi-10 locus in rice breeding programs and will also be
useful as genetic markers for high resolution mapping of the Pi-10 region.