Reference "Screening of RAPD markers linked to the photoperiod-sensitivity gene in rice chromosome 6 using bulked segregant analysis"

Reference ID

2889

Title

Screening of RAPD markers linked to the photoperiod-sensitivity gene in rice chromosome 6 using bulked segregant analysis

Source

DNA research : an international journal for rapid publication of reports on genes and genomes, 1995, vol. 2, pp. 101-106

Authors (5)

Monna-L	Miyao-A
Zhong-H-S	Sasaki-T
Minobe-Y

Abstract

Bulked segregant analysis was used to determine randomly amplified polymorphic
DNA (RAPD) markers in a specific interval in the middle of chromosome 6 of rice
for tagging the photoperiod sensitivity gene. Two pools of F2 individuals
(japonica cv. Nipponbare and indica cv. Kasalath) were constructed according to
the genotypes of three restriction fragment length polymorphism (RFLP) markers
located at both ends and the middle of the targeted interval. Then another pair
of pools were constructed based on the "graphical genotype," which was made
with our high density linkage map. RAPD analysis was performed using these DNA
pools as templates, and polymorphic fragments were detected and mapped. Using
80 primers, either singly or pairwise, we tested 2,404 primer pairs and
established 14 markers tightly linked to the photoperiod sensitivity gene. The
obtained RAPD markers were converted into sequence-tagged sites by cloning and
sequencing of the polymorphic fragments and they can be used directly for
construction of physical maps. This bulked segregant method can be applied for
any species and any region of interest in which detailed linkage maps or
physical maps are needed.