Reference "Two rice MADS domain proteins interact with OsMADS1"

Reference ID

2451

Title

Two rice MADS domain proteins interact with OsMADS1

Source

Plant molecular biology, 2000, vol. 44, pp. 513-527

Authors (4)

Lim-J	Moon-Y-H
An-G	Jang-S-K

Abstract

OsMADS1 is a MADS box gene controlling flower development in rice. In order to
learn more about the function of OsMADS1, we searched for cellular proteins
interacting with OsMADS1 employing the yeast two- hybrid system. Two novel
proteins with MADS domains, which were named OsMADS14 and OsMADS15, were
isolated from a rice cDNA library. OsMADS14 and -15 are highly homologous to the
maize MADS box gene ZAP1 which is an orthologue of the floral homeotic gene
APETALA1 (AP1). Interactions among the three MADS domain proteins were confirmed
by in vitro experiments using GST-fused OsMADS1 expressed in Escherichia coli
and in vitro translated proteins of OsMADS14 and -15. We determined which
domains in OsMADS1, -14, and -15 were required for protein-protein interaction
employing the two-hybrid system and pull-down experiments. While the K domain
was essential for protein-protein interaction, a region preceded by the K domain
augmented this interaction. Interestingly, the C-terminal region of OsMADS1
functioned as a transcriptional activation domain in yeast and mammalian cells,
while, on the other hand, the C domains of OsMADS14 and -15 exhibited only very
weak transcriptional activator functionality, if any at all.