Reference "Isolation, characterization and mRNA expression of four cDNAs encoding translation elongation factor 1A from rice (Oryza sativa L.)"

Reference ID

2034

Title

Isolation, characterization and mRNA expression of four cDNAs encoding translation elongation factor 1A from rice (Oryza sativa L.)

Source

Plant molecular biology, 1998, vol. 36, pp. 137-148

Authors (2)

Kidou-S

Ejiri-S

Abstract

Four different cDNA clones encoding protein synthesis elongation factor 1A,
eEF1A, were isolated from rice (Oryza sativa L.). The genes encoded by these
cDNAs were designated rice elongation factor 1A genes refa1, refa2, refa3 and
refa4. The genes encoded identical eEF-1A polypeptides and shared high amino
acid identity with eEF1A of other eukaryotes. Southern blot analysis suggested
that some of these refa genes may be organized in a cluster on the same
chromosome within a short distance. PCR analysis of rice genomic DNA showed that
refa1 and refa4, and refa3 and refa2 are in neighboring locations on the rice
genome. The mRNAs of the four refa genes accumulated to nearly equal levels in a
variety of tissues and at different stages of growth. Suspension-cultured cells
were the most abundant in refa mRNAs. Dormant seeds contained a small amount of
the four refa mRNAs. Transcript accumulation was highly induced after seed
germination, and the same expression levels were maintained even in old leaf
blades of mature plants.