Reference "Cytochrome oxidase subunit II gene of rice has an insertion sequence within the intron"

Reference ID

1913

Title

Cytochrome oxidase subunit II gene of rice has an insertion sequence within the intron

Source

Nucleic acids research, 1984, vol. 12, pp. 7305-7315

Authors (3)

Kao-T	Moon-E
Wu-R

Abstract

We have isolated and sequenced the cytochrome oxidase subunit II gene from rice
(Oryza sativa L. var Labelle). The overall structural organization of this gene
is very similar to that of the maize gene. This gene contains an intron in a
position identical to the intron in the maize gene. However, the intron in the
rice gene is longer than that of the maize gene largely due to a 461 bp
insertion sequence, which has inverted repeats at its termini and is flanked by
direct repeats, characteristic of transposable elements. Apart from this
insertion sequence, the remainder of the intron sequence is strikingly
homologous to that of maize (98.6% homology), suggesting a possible functional
or structural role. The coding regions of the two genes exhibit 99.5% nucleotide
sequence homology and their deduced amino acid sequences are identical.
Similarly, the 3'-noncoding regions, except for several small insertions and
deletions, show complete sequence homology. On the contrary, no sequence
homology is detected in the 5'-noncoding regions.