Reference "A chimeric gene containing the 5' portion of atp6 is associated with cytoplasmic male-sterility of rice"

Reference ID

1831

Title

A chimeric gene containing the 5' portion of atp6 is associated with cytoplasmic male-sterility of rice

Source

Molecular & General Genetics, 1990, vol. 224, pp. 10-16

Authors (3)

Kadowaki-K	Suzuki-T
Kazama-S

Abstract

Three ATPase subunit 6 (atp6) genes of rice mitochondria were isolated, one from
normal and two from cms-Bo male-sterile cytoplasms, in order to determine
whether the extra atp6 gene in cms-Bo rice plays a role in cytoplasmic male-
sterility (CMS). The nucleotide sequences of all three genes were determined and
analysis showed a chimeric atp6 gene (urf- rmc) as well as a normal atp6 gene in
cms-Bo cytoplasm, but only the normal atp6 gene in normal cytoplasm. The urf-rmc
gene is completely homologous to the normal atp6 gene from at least position -
426 in the 5' flanking region to position +511 downstream from the initiation
codon ATG: however, the following downstream sequence shows no homology with the
normal rice atp6 gene, or any other reported sequence. Introduction of the
restorer of fertility gene altered transcription of the urf-rmc gene but not the
atp6 gene, indicating participation of the chimeric gene in the expression of
CMS. Southern blot analysis showed that the urf-rmc gene was generated by an
intramolecular recombination event in mitochondrial DNA, and the homologous
recombination point between the atp6 gene and the opposite ancestral sequence
was identified as 5'-TTCCCTC-3'.