Reference "Analysis of intragenic recombination at wx in rice: correlation between the molecular and genetic maps within the locus"

Reference ID

1623

Title

Analysis of intragenic recombination at wx in rice: correlation between the molecular and genetic maps within the locus

Source

Genome ( National Research Council Canada ), 2000, vol. 43, pp. 589-596

Authors (4)

Inukai-T	Sako-A
Hirano-H-Y	Sano-Y

Abstract

In plant genomes as well as other eukaryotic genomes, meiotic recombination does
not occur uniformly. At the level of the gene, high recombination frequencies
are often observed within genetic loci in maize, but this feature of intragenic
recombination is not seen at the csr1 locus in Arabidopsis. These observations
suggest that meiotic recombination in plant genomes varies considerably among
species. In the present study we investigated meiotic recombination at the wx
locus in rice. The mutation sites of wx mutants induced by ethyl
methanesulfonate (EMS) treatment or gamma-ray irradiation and a spontaneous wx
mutant were physically characterized, and the genetic distances between those wx
mutation sites were estimated by pollen analysis. Based on these results, the
recombination frequency at the wx locus in rice was estimated as 27.3 kb/cM,
which was about 10 times higher than the average for the genome, suggesting that
there was a radically different rate of meiotic recombination for intra- and
intergenic regions in the rice genome.