Reference "Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L"

Reference ID

1566

Title

Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L

Source

Plant molecular biology, 1997, vol. 33, pp. 857-865

Authors (6)

Igarashi-Y	Yoshiba-Y
Sanada-Y	Yamaguchi-Shinozaki-K
Wada-K	Shinozaki-K

Abstract

A cDNA for delta1-pyrroline-5-carboxylate (P5C) synthetase (cOsP5CS), an enzyme
involved in the biosynthesis of proline, was isolated and characterized from a
cDNA library prepared from 14-day-old seedlings of Oryza sativa cv. Akibare. The
deduced amino acid sequence of the P5CS protein (OsP5CS) from O. sativa
exhibited 74.2% and 75.5% homology to that of the P5CS from Arabidopsis thaliana
and Vigna aconitifolia, respectively. Northern blot analysis revealed that the
gene for P5CS (OsP5CS) was induced by high salt, dehydration, treatment of ABA
and cold treatment, while it was not induced by heat treatment. Simultaneously,
accumulation of proline was observed as a result of high salt treatment in O.
sativa. Moreover, the levels of expression of OsP5CS mRNA and content of proline
under salt stress condition were compared between a salt-tolerant cultivar, Dee-gee-woo-
gen (DGWG) and a salt-sensitive breeding line, IR28. It was observed that the
expression of the P5CS gene and the accumulation of proline in DGWG steadily
increased, whereas those in IR28 increased slightly.