Reference "Promoter structure and activity of type 1 rice metallothionein-like gene"

Reference ID

1502

Title

Promoter structure and activity of type 1 rice metallothionein-like gene

Source

DNA sequence : the journal of DNA sequencing and mapping, 1998, vol. 9, pp. 9-17

Authors (2)

Hsieh-H-M

Huang-P-C

Abstract

A novel stress-inducible metallothionein-like gene from rice, designated as rgMT-
1 (rice genomic metallothionein-like gene-1), was isolated and sequenced. From
the sequence analysis of its 5'-flanking region, two putative TATA boxes, one
CAAT box, and several short sequences homologous to regulatory cis-elements
previously reported were identified. Two direct repeats, one 10 bp in length
(CAAAATCAAA) and the other 11 bp (GTGAAAATACT), respectively, were also found.
By transient GUS (beta-glucuronidase) assay, the expression of GUS, in vitro,
was enhanced by the presence of the rgMT-1 intron. The critical region which
controls the basal transcription was shown to lie between - 73 and -36 upstream
of rgMT-1, in which one of the two putative TATA boxes was located. The promoter
activity was lost completely when both putative TATA boxes were deleted. This is
the first report describing the genomic structure and regulation of a
monocotyledonous metallothionein-like gene critical to the response of stress.