Reference "Isolation and characterization of cDNA clones encoding cdc2 homologues from Oryza sativa: a functional homologue and cognate variants"

Reference ID

1324

Title

Isolation and characterization of cDNA clones encoding cdc2 homologues from Oryza sativa: a functional homologue and cognate variants

Source

Molecular & General Genetics, 1992, vol. 233, pp. 10-16

Authors (9)

Hashimoto-J	Hirabayashi-T
Hayano-Y	Hata-S
Ohashi-Y	Suzuka-I
Utsugi-T	Toh-e-A
Kikuchi-Y

Abstract

Using probes obtained by PCR amplification, we have isolated two cognate rice
cDNAs (cdc2Os-1 and cdc2Os-2) encoding structural homologues of the cdc2+/CDC28
(cdc2) protein kinase from a cDNA library prepared from cultured rice cells.
Comparison of the deduced amino acid sequences of cdc2Os-1 and cdc2Os-2 showed
that they are 83% identical. They are 62% identical to CDC28 of Saccharomyces
cerevisiae and much more similar to the yeast and mammalian p34cdc2 kinases than
to rice R2, a cdc2-related kinase isolated previously by screening the same rice
cDNA library with a different oligonucleotide probe. Southern blot analysis
indicated that the three rice clones (cdc2Os-1, cdc2Os-2 and R2) are derived
from distinct genes and are each found in a single copy per rice haploid genome.
RNA blot analysis revealed that these genes are expressed in proliferating rice
cells and in young rice seedlings. cdc2Os-1 could complement a temperature-
sensitive yeast mutant of cdc28. However, despite the similarity in structure,
both cdc2Os-2 and R2 were unable to complement the same mutant. Thus, the
present results demonstrate the presence of structurally related, but
functionally distinct cognates of the cdc2 cell cycle kinase in rice.