Reference "A high-density rice genetic linkage map with 2275 markers using a single F2 population"

Reference ID

1313

Title

A high-density rice genetic linkage map with 2275 markers using a single F2 population

Source

Genetics, 1998, vol. 148, pp. 479-494

Authors (17)

Harushima-Y	Yano-M	Shomura-A	Sato-M	Shimano-T
Kuboki-Y	Yamamoto-T	Lin-S-Y	Antonio-B-A	Parco-A
Kajiya-H	Huang-N	Yamamoto-K	Nagamura-Y	Kurata-N
Khush-G-S	Sasaki-T

Abstract

A 2275-marker genetic map of rice (Oryza sativa L.) covering 1521.6 cM in the
Kosambi function has been constructed using 186 F2 plants from a single cross
between the japonica variety Nipponbare and the indica variety Kasalath. The map
provides the most detailed and informative genetic map of any plant. Centromere
locations on 12 linkage groups were determined by dosage analysis of secondary
and telotrisomics using > 130 DNA markers located on respective chromosome arms.
A limited influence on meiotic recombination inhibition by the centromere in the
genetic map was discussed. The main sources of the markers in this map were
expressed sequence tag (EST) clones from Nipponbare callus, root, and shoot
libraries. We mapped 1455 loci using ESTs; 615 of these loci showed significant
similarities to known genes, including single-copy genes, family genes, and
isozyme genes. The high-resolution genetic map permitted us to characterize
meiotic recombinations in the whole genome. Positive interference of meiotic
recombination was detected both by the distribution of recombination number per
each chromosome and by the distribution of double crossover interval lengths.