Reference "Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor"

Reference ID

11686

Title

Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor

Source

The Plant journal : for cell and molecular biology, 2004, vol. 37, pp. 1-8

Authors (17)

Cho-E-M	Okada-A	Kenmoku-H	Otomo-K	Toyomasu-T
Mitsuhashi-W	Sassa-T	Yajima-A	Yabuta-G	Mori-K
Oikawa-H	Toshima-H	Shibuya-N	Nojiri-H	Omori-T
Nishiyama-M	Yamane-H

Abstract

We have isolated and characterized a cDNA encoding a novel diterpene cyclase,
OsDTC1, from suspension-cultured rice cells treated with a chitin elicitor.
OsDTC1 functions as ent-cassa-12,15-diene synthase, which is considered to play
a key role in the biosynthesis of (-)-phytocassanes recently isolated as rice
diterpenoid phytoalexins. The expression of OsDTC1 mRNA was also confirmed in
ultraviolet (UV)-irradiated rice leaves. In addition, we identified ent-cassa-12,15-
diene, a putative diterpene hydrocarbon precursor of (-)-phytocassanes, as an
endogenous compound in the chitin-elicited suspension-cultured rice cells and
the UV-irradiated rice leaves. The OsDTC1 cDNA isolated here will be a useful
tool to investigate the regulatory mechanisms of the biosynthesis of (-)-
phytocassanes in rice.