Reference "3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase is regulated for the accumulation of polysaccharide-linked hydroxycinnamoyl esters in rice (Oryza sativa L.) internode cell walls"

Reference ID

11668

Title

3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase is regulated for the accumulation of polysaccharide-linked hydroxycinnamoyl esters in rice (Oryza sativa L.) internode cell walls

Source

Plant Cell Rep, 2006, vol. 25, pp. 676-688

Authors (10)

Sato-K	Mase-K
Nakano-Y	Nishikubo-N
Sugita-R	Tsuboi-Y
Kajita-S	Zhou-J
Kitano-H	Katayama-Y

Abstract

Polysaccharide-linked hydroxycinnamoyl esters (PHEs) over-accumulate in the
internodes of a rice (Oryza sativa L.) mutant, Fukei 71 (F71). This accumulation
is accompanied by over-expression of phenylalanine ammonialyase (PAL). In this
study, we show that only one member of the 3-deoxy-D-arabino-heptulosonate 7-
phosphate synthase (DAHPS) family expresses in close correlation with PAL.
Furthermore, substrate availability to DAHPS is promoted by down-regulating the
expression of plastidic pyruvate kinase (PKp), a competitor of DAHPS. Since the
over-production of PHEs is caused by D50 gene disruption, these results suggest
that specific enzymes in the phenylpropanoid and shikimate pathways are
coordinately up-regulated. In addition, the results indicate that carbon-flow
into the shikimate pathway is modified for the synthesis of PHEs, and is
probably controlled by D50.