Reference "OsDMC1 is required for homologous pairing in Oryza sativa"

Reference ID

11620

Title

OsDMC1 is required for homologous pairing in Oryza sativa

Source

Plant molecular biology, 2007, vol. , pp. -

Authors (2)

Deng-Z-Y

Wang-T

Abstract

OsDMC1 is the rice homologue of the yeast DMC1 gene. Here, we analyzed the
function of OsDMC1 in meiosis using an RNA interference approach. The OsDMC1-
RNAi lines grew normally during their vegetative phase but showed spikelet and
pollen sterility. The sterility phenotypes were associated with down-regulated
OsDMC1 transcript and protein levels mediated by RNAi. Further cytological
observations of male meiocytes revealed that knock-down of OsDMC1 led to defects
in bivalent formation and subsequent unequal chromosome segregation and
irregular spore generation, and induced changes in male meiotic progression.
Fluorescent in situ hybridization experiments revealed that the OsDMC1-RNAi
lines were defective in homologous pairing. These data indicate that OsDMC1 is
essential for rice meiosis and plays an important role in homologous pairing.