Reference "Fine mapping of pss1, a pollen semi-sterile gene in rice (Oryza sativa L.)"

Reference ID

11496

Title

Fine mapping of pss1, a pollen semi-sterile gene in rice (Oryza sativa L.)

Source

Theor Appl Genet, 2007, vol. , pp. -

Authors (8)

Li-W	Jiang-L
Zhou-S	Wang-C
Liu-L	Chen-L
Ikehashi-H	Wan-J

Abstract

During routine seed increase procedures in rice, semi-sterile plants are common;
however, such semi-sterility mutants in rice varieties have been only rarely
analyzed genetically. W207-2 is a semi-sterile selection from the japonica rice
variety Nipponbare. In this report, we found the female gamete of W207-2 was
normal, and its semi-sterility was unaffected by growth duration but was
conditioned by a recessive nuclear gene whose action leads to pollen semi-
sterility and anther indehiscence, and the gene was named as pss1 (pollen semi-
sterile). Using an F(2) population derived from the two parents W207-2 and Dular
and a pooled DNA strategy, pss1 was mapped to an interval on chromosome 8
defined by the two SSR loci RM6356 and RS41. The position of pss1 was confirmed
in another F(2) population derived from the cross W207-2 x Nipponbare. Over
2,000 homozygous pss1 segregants from the large W207-2 x Dular F(2) population
were used to fine map pss1 to a 0.04 cM segment flanked by a CAPs marker L2 and
a dCAPs L3 marker. Sequences for both markers are present on a single PAC clone,
and the physical distance between them is about 28 kb. Analysis of the PAC
sequence predicts the presence of five open reading frames, they are as follows:
putative ribonuclease PH, putative avr9 elicitor response protein, kinesin1-like
protein, putative protein RNP-D precursor and putative 40S ribosomal protein
S13. This result would be helpful in cloning the pss1 gene.