Reference "A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice"

Reference ID

11474

Title

A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice

Source

Plant & cell physiology, 2005, vol. 46, pp. 1674-1681

Authors (4)

Xu-M	Zhu-L
Shou-H	Wu-P

Abstract

Auxin transport affects a variety of important growth and developmental
processes in plants, including the regulation of shoot and root branching. The
asymmetrical localization of auxin influx and efflux carriers within the plasma
membrane establishes the auxin gradient and facilitates its transport. REH1, a
rice EIR1 (Arabidopsis ethylene insensitive root 1)-like gene, is a putative
auxin efflux carrier. Phylogenetic analysis of 32 members of the PIN family,
taken from across different species, showed that in terms of evolutionary
relationship, OsPIN1 is closer to the PIN1 family than to the PIN2 family. It
is, therefore, renamed as OsPIN1 in this study. OsPIN1 was expressed in the
vascular tissues and root primordial in a manner similar to AtPIN1. Adventitious
root emergence and development were significantly inhibited in the OsPIN1 RNA
interference (RNAi) transgenic plants, which was similar to the phenotype of NPA
(N-1-naphthylphalamic acid, an auxin-transport inhibitor)-treated wild-type
plants. alpha-naphthylacetic acid (alpha-NAA) treatment was able to rescue the
mutated phenotypes occurring in the RNAi plants. Overexpression or suppression
of the OsPIN1 expression through a transgenic approach resulted in changes of
tiller numbers and shoot/root ratio. Taken together, these data suggest that
OsPIN1 plays an important role in auxin-dependent adventitious root emergence
and tillering.