Reference "Isolation of cold stress-responsive genes in the reproductive organs, and characterization of the OsLti6b gene from rice (Oryza sativa L.)"

Reference ID

11450

Title

Isolation of cold stress-responsive genes in the reproductive organs, and characterization of the OsLti6b gene from rice (Oryza sativa L.)

Source

Plant Cell Rep, 2007, vol. , pp. -

Authors (6)

Kim-S-H	Kim-J-Y
Kim-S-J	An-K-S
An-G	Kim-S-R

Abstract

During their reproductive stage, rice crops often are exposed to cold stress,
which leads to sterility and reduced yields. To understand the cold response
mechanism at that stage, we used an mRNA differential display method to isolate
cold-responsive genes from pre-anthesis flowers. Approximately 5,000 transcripts
were identified here, of which 123 were found to be displayed differentially
between the control (30 degrees C) and cold-treated (12 degrees C) flowers.
Among them, 26 were analyzed by northern analysis; 8 of those clones were
confirmed as cold-responsive. OsLti6b, encoding a hydrophobic protein homologous
to Arabidopsis RCI2, was analyzed in detail. RNA blot analysis revealed that its
transcript is increased by cold, salt, drought, or ABA treatments. In situ
hybridization indicated that this transcript is highly accumulated in the
ovaries and stamens of cold-treated flowers, particularly in the anther walls
and vascular tissues of the filaments. Over-expression of OsLti6b increased cold
tolerance as revealed by seedling wilting rates and ion leakages of mature
leaves, demonstrating that the extent of the tolerance correlates well with its
expression level.