Reference "A perfect marker for fragrance genotyping in rice"

Reference ID

11434

Title

A perfect marker for fragrance genotyping in rice

Source

Molecular Breeding, 2005, vol. 16, pp. 279-283

Authors (5)

Bradbury-L-M-T	Henry-J-H
Jin-Q	Reinke-R-F
Waters-D-L-E

Abstract

Allele specific amplification (ASA) is a low-cost, robust technique that can be
utilised to discriminate between alleles that differ by SNP's, insertions or
deletions, within a single PCR tube. Fragrance in rice, a recessive trait, has
been shown to be due to an eight bp deletion and three SNP's in a gene on
chromosome 8 which encodes a putative betaine aldehyde dehydrogenase 2 (BAD2).
Here we report a single tube ASA assay which allows discrimination between
fragrant and non-fragrant rice varieties and identifies homozygous fragrant,
homozygous non-fragrant and heterozygous non-fragrant individuals in a
population segregating for fragrance. External primers generate a fragment of
approximately 580 bp as a positive control for each sample. Internal and
corresponding external primers produce a 355 bp fragment from a non-fragrant
allele and a 257 bp fragment from a fragrant allele, allowing simple analysis on
agarose gels.