Reference "OsWRKY71, a rice transcription factor, is involved in rice defense response"

Reference ID

11287

Title

OsWRKY71, a rice transcription factor, is involved in rice defense response

Source

J Plant Physiol, 2006, vol. , pp. -

Authors (4)

Liu-X	Bai-X
Wang-X	Chu-C

Abstract

WRKY proteins are a large family of transcription factors that mainly
participate in plant biotic stress responses. So far, one hundred and five
OsWRKY genes have been predicted in the rice genome. To identify OsWRKY genes
that might function in inducible defense responses, a phylogenetic tree
including 184 WRKY proteins from Arabidopsis thaliana, rice, and other species
was constructed. Based on the phylogenetic analysis, ten candidate OsWRKY genes
that may be involved in defense responses were isolated from salicylic acid (SA)-
treated rice seedlings. One of them, OsWRKY71, was up-regulated by several
defense signaling molecules, such as SA, methyl jasmonate (MeJA), 1-aminocyclo-propane-1-
carboxylic acid (ACC), as well as wounding and pathogen infection, suggesting
that OsWRKY71 might function in rice biotic stress response. Transient
expression of OsWRKY71:GFP fusion protein in onion epidermis cells revealed that
OsWRKY71 was localized in the nucleus. Overexpression of OsWRKY71 gene in rice
resulted in enhanced resistance to virulent bacterial pathogens Xanthomonas
oryzae pv. oryzae (Xoo) 13751. Furthermore, two marker genes in defense
signaling pathway, OsNPR1 and OsPR1b, were constitutively expressed in OsWRKY71-
overexpressing transgenic plants. These results suggest that OsWRKY71 might
function as a transcriptional regulator upstream of OsNPR1 and OsPR1b in rice
defense signaling pathways.