Reference "Mutations in the eIF(iso)4G translation initiation factor confer high resistance of rice to Rice yellow mottle virus"

Reference ID

11146

Title

Mutations in the eIF(iso)4G translation initiation factor confer high resistance of rice to Rice yellow mottle virus

Source

The Plant journal : for cell and molecular biology, 2006, vol. , pp. -

Authors (6)

Albar-L	Bangratz-Reyser-M
Hebrard-E	Ndjiondjop-M-N
Jones-M	Ghesquiere-A

Abstract

We report here evidence of the role that the isoform of the eukaryotic
translation initiation factor 4G (eIF(iso)4G) plays in naturally occurring
resistance in plant/virus interactions. A genetic and physical mapping approach
was developed to isolate the Rymv1 locus controlling the high recessive
resistance to Rice yellow mottle virus (RYMV) in the rice (Oryza sativa) variety
Gigante. The locus was mapped to a 160-kb interval containing a gene from the
eIF(iso)4G family. The stable transformation of a resistant line with the cDNA
of this gene, derived from a susceptible variety, resulted in the loss of
resistance in transgenic plants. The allelic variability of this gene was
analysed in three resistant and 17 susceptible varieties from different
cultivated rice species or subspecies. Compared with susceptible varieties,
resistant varieties present specific alleles, characterized by either amino acid
substitutions or short amino-acid deletions in the middle domain of the protein.
The structure of this domain was modelled and showed that the substitutions were
clustered on a small surface patch. This suggests that this domain may be
involved in an interaction with the virus.