Reference "Two rice DMC1 genes are differentially expressed during meiosis and during haploid and diploid mitosis"

Reference ID

11123

Title

Two rice DMC1 genes are differentially expressed during meiosis and during haploid and diploid mitosis

Source

Sexual Plant Reproduction, 2002, vol. 14, pp. 257-267

Authors (3)

Kathiresan-A	Khush-G-S
Bennett-J

Abstract

We have cloned two rice homologues of yeast DMC1, a meiosis-specific gene
required for recombination between homologous chromosomes. We show that rice
DMC1A and DMC1B were produced by a gene duplication event that occurred after
rice separated from the common ancestor of the cereals. The predicted proteins
contain 344 amino acids, of which all but 7 are conserved between the two
homologues. Between bases -1 and -245, the two promoters share six invariant
blocks of sequence of 10-28 bp, interspersed in variable sequences. Both DMC1A
and DMC1B are expressed in pollen mother cells coincident with meiosis, and in
diploid non-meiotic tissues such as calli and root tips. DMC1B is also expressed
in haploid male gametophytes during pollen maturation and in diploid zygotic
embryos and endosperm after pollination. These data suggest that DMC1B, either
alone or in combination with DMC1A, contributes to recombination during meiosis
and during haploid and diploid mitosis.