Trait Name |
Trait Protocol |
Trait Values |
FT10 |
Plants were checked bi-weekly for presence of first buds, and the average flowering time of 4 plants of the same accession were collected; 10 degC, 16 hrs daylight |
|
FT16 |
Plants were checked bi-weekly for presence of first buds, and the average flowering time of 4 plants of the same accession were collected; 16 degC, 16 hrs daylight |
|
FT22 |
Plants were checked bi-weekly for presence of first buds, and the average flowering time of 4 plants of the same accession were collected; 22 degC, 16 hrs daylight |
|
Seedling Growth |
Seedling growth rate was given by leaf area divided by the number of days of growth since sowing ( in cm2/day); Seeds were grown for one week in the greenhouse under long day (16 hours light) |
|
Vern Growth |
Vegetative growth rate during vernalization was estimated as the increment of cm2 leaf area per day between each of the three time points for which leaf area was measured; Seeds were grown for one week in the greenhouse under long day (16 hours light), vernalized for 4 weeks (4 degC, 16h light, 50% relative humidity) |
|
After Vern Growth |
Vegetative growth rate after vernalization was estimated as the increment of cm2 leaf area per day between each of the three time points for which leaf area was measured; Seeds were grown for one week in the greenhouse under long day (16 hours light), vernalized for 4 weeks (4 degC, 16h light, 50% relative humidity)and then returned to greenhouse |
|
Secondary Dormancy |
Secondary dormancy was given by the slope between the germination percentages of non-dormant seeds after one and six weeks of cold treatment. Viability of non germinating seeds after cold treatment was confirmed as described in Cadman et al., 2006; Fully after-ripened seeds were treated with a 1- and 6-week long exposure to 4 degC |
|
Germ in dark |
The ability to germinate in the dark at 4 degC was measured as the percentage of non dormant seeds that can germinate during 1-week long cold exposure, in the absence of light; 4 degC, in the dark |
|
DSDS50 |
Number of days of seed dry storage required to reach 50% germination, or DSDS50 value (Alonso-Blanco et al., 2003); Dry storage, followed by 25 degC, 12h day, 20 degC, 12h night for 1 week |
|
Seed bank 133-91 |
Genotypes consistently decreased their percentage of germination between 91 and 133 days of dry storage. The time point for this sudden reduction in germination rate was scored by the slope between germination percentages at these two time points; Between 91 and 133 days of dry storage |
|
Storage 7 days |
Primary dormancy was measured as the progressive increase of germination rate measured after 7 days of dry storage; 7 days dry storage as described in Alonso-Blanco et al, 2003 |
|
Storage 28 days |
Primary dormancy was measured as the progressive increase of germination rate measured after 28 days of dry storage; 28 days dry storage as described in Alonso-Blanco et al, 2003 |
|
Storage 56 days |
Primary dormancy was measured as the progressive increase of germination rate measured after 56 days of dry storage; 56 days dry storage as described in Alonso-Blanco et al, 2003 |
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