| Trait Name |
Trait Protocol |
Trait Values |
| LD |
Number of days following stratification to opening of first flower. The experiment was stopped at 200 d, and accessions that had not flowered at that point were assigned a value of 200; 18 degC, 16 hrs daylight |
|
| LDV |
Number of days following stratification to opening of first flower. The experiment was stopped at 200 d, and accessions that had not flowered at that point were assigned a value of 200; 18 degC, 16 hrs daylight, vernalized (5 wks, 4 degC) |
|
| SD |
Number of days following stratification to opening of first flower. The experiment was stopped at 200 d, and accessions that had not flowered at that point were assigned a value of 200; 18 degC, 8 hrs daylight |
|
| SDV |
Number of days following stratification to opening of first flower. The experiment was stopped at 200 d, and accessions that had not flowered at that point were assigned a value of 200; 18 degC, 8 hrs daylight, vernalized (5 wks, 4 degC) |
|
| FT10 |
Plants were checked bi-weekly for presence of first buds, and the average flowering time of 4 plants of the same accession were collected; 10 degC, 16 hrs daylight |
|
| FT16 |
Plants were checked bi-weekly for presence of first buds, and the average flowering time of 4 plants of the same accession were collected; 16 degC, 16 hrs daylight |
|
| FT22 |
Plants were checked bi-weekly for presence of first buds, and the average flowering time of 4 plants of the same accession were collected; 22 degC, 16 hrs daylight |
|
| Seed Dormancy |
Number of days of seed dry storage required to reach 50% germination, or DSDS50 value (Alonso-Blanco et al., 2003). The measurement for each genotype was calculated as the average value across all available replicates; Seeds stored in cellulose paper bags and kept in a dark incubator at 20 degC, 40% relative humidity |
|
| Emco5 |
All interactions were scored specifically on first true leaves as compatible, incompatible or intermediate depending on the consistency of presence / absence of sporangiophores determined on 5-10 seedlings of each genotype with three independent replications; 20-22 degC, 10 hrs daylight, 70% humidity |
|
| Emwa1 |
All interactions were scored specifically on first true leaves as compatible, incompatible or intermediate depending on the consistency of presence / absence of sporangiophores determined on 5-10 seedlings of each genotype with three independent replications; 20-22 degC, 10 hrs daylight, 70% humidity |
|
| Emoy* |
All interactions were scored specifically on first true leaves as compatible, incompatible or intermediate depending on the consistency of presence / absence of sporangiophores determined on 5-10 seedlings of each genotype with three independent replications; 20-22 degC, 10 hrs daylight, 70% humidity |
|
| Hiks1 |
All interactions were scored specifically on first true leaves as compatible, incompatible or intermediate depending on the consistency of presence / absence of sporangiophores determined on 5-10 seedlings of each genotype with three independent replications; 20-22 degC, 10 hrs daylight, 70% humidity |
|
| Noco2 |
All interactions were scored specifically on first true leaves as compatible, incompatible or intermediate depending on the consistency of presence / absence of sporangiophores determined on 5-10 seedlings of each genotype with three independent replications; 20-22 degC, 10 hrs daylight, 70% humidity |
|
| Li7 |
Lithium concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| B11 |
Boron concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Na23 |
Sodium concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Mg25 |
Magnesium concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| P31 |
Phosphorus concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| S34 |
Sulfur concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| K39 |
Potassium concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Ca43 |
Calcium concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Mn55 |
Manganese concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Fe56 |
Iron concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Co59 |
Cobalt concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Ni60 |
Nickel concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Cu65 |
Copper concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Zn66 |
Zinc concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| As75 |
Arsenic concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Se82 |
Selenium concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Mo98 |
Molybdenum concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| Cd114 |
Cadmium concentrations in leaves, grown in soil. Elemental analysis was performed with an ICP-MS (PerkinElmer). Sample normalized to calculated weights as described in Baxter et al., 2008; 20 degC, 16 hrs daylight |
|
| avrPphB |
Following inoculation of two leaves per plant with 0.1 ml of 10 -8 cfu/ml bacteria in 10 mM MgSO4 buffer using a blunt-tipped syringe, leaf collapse was scored at 20 hrs and again at 24 hrs after inoculation. A positive score at either time point was deemed a hypersensitive response; 20 degC, 12 hrs daylight |
|
| avrRpm1 |
Following inoculation of two leaves per plant with 0.1 ml of 10 -8 cfu/ml bacteria in 10 mM MgSO4 buffer using a blunt-tipped syringe, leaf collapse was scored at 20 hrs and again at 24 hrs after inoculation. A positive score at either time point was deemed a hypersensitive response; 20 degC, 12 hrs daylight |
|
| avrRpt2 |
Following inoculation of two leaves per plant with 0.1 ml of 10 -8 cfu/ml bacteria in 10 mM MgSO4 buffer using a blunt-tipped syringe, leaf collapse was scored at 20 hrs and again at 24 hrs after inoculation. A positive score at either time point was deemed a hypersensitive response; 20 degC, 12 hrs daylight |
|
| avrB |
Following inoculation of two leaves per plant with 0.1 ml of 10 -8 cfu/ml bacteria in 10 mM MgSO4 buffer using a blunt-tipped syringe, leaf collapse was scored at 20 hrs and again at 24 hrs after inoculation. A positive score at either time point was deemed a hypersensitive response; 20 degC, 12 hrs daylight |
|
| 0W |
Number of days required for the bolt height to reach 5cm; 23 degC, 16hrs daylight |
|
| 2W |
Number of days required for the bolt height to reach 5cm; 23 degC, 16hrs daylight. Vernalized for 2 wks at 5 degC, 8hrs daylight |
|
| 4W |
Number of days required for the bolt height to reach 5cm; 23 degC, 16hrs daylight. Vernalized for 4 wks at 5 degC, 8hrs daylight |
|
| 8W |
Number of days required for the bolt height to reach 5cm; 23 degC, 16hrs daylight. Vernalized for 8 wks at 5 degC, 8hrs daylight |
|
| FLC |
RNA was extracted from leaves after 4 wks of growth. FLC gene expression levels were determined by Northern hybridization quantified relative to $Beta;-TUBULIN expression; Growth in greenhouse, 20-22 degC, 16hrs daylight |
|
| FRI |
RNA was extracted from leaves after 4 wks of growth. FRI gene expression levels were determined by Northern hybridization quantified relative to $Beta;-TUBULIN expression; Growth in greenhouse, 20-22 degC, 16hrs daylight |
|
| 8W GH FT |
Flowering time was scored as the number of days for the bolt to reach 5cm; 20-22 degC, natural light from the middle of October 2002 till March 2003, vernalized for 8wks at 4 degC, 8hrs daylight |
|
| 8W GH LN |
Leaf number was scored as the number of rosette leaves and the number of cauline leaves when the bolt reached 5cm; 20-22 degC, natural light from the middle of October 2002 till March 2003, vernalized for 8wks at 4 degC, 8hrs daylight |
|
| 0W GH FT |
Flowering time was scored as the number of days for the bolt to reach 5cm; 20-22 degC, natural light from the middle of October 2002 till March 2003 |
|
| 0W GH LN |
Leaf number was scored as the number of rosette leaves and the number of cauline leaves when the bolt reached 5cm; 20-22 degC, natural light from the middle of October 2002 till March 2003 |
|
| FT Field |
Flowering time was scored as the number of days between germination date and appearance of the first flower; Growth in field, natural light, started in October |
|
| FT Diameter Field |
Longest diameter of the plant measured on the day the first flower was observed; Growth in field, natural light, started in October |
|
| FT GH |
Flowering time was scored as the number of days between germination date and appearance of the first flower; Growth in greenhouse, 20 degC, 16hrs daylight |
|
| FT Duration GH |
Number of days between appearance of the first flower and the senescence of the last flower; Growth in greenhouse, 20 degC, 16hrs daylight |
|
| LC Duration GH |
Number of days between germination and plant complete senescence; Growth in greenhouse, 20 degC, 16hrs daylight |
|
| LFS GH |
Number of days between germination and senescence of the last flower; Growth in greenhouse, 20 degC, 16hrs daylight |
|
| MT GH |
Number of days between last flower senescence and complete plant senescence; Growth in greenhouse, 20 degC, 16hrs daylight |
|
| RP GH |
Reproduction period: number of days between the appearance of the first flower and the plant complete senescence; Growth in greenhouse, 20 degC, 16hrs daylight |
|
| At1 |
Four days after inoculation, leaves were scored by eye for disease symptom using a scale from 0 (no visible symptom) to 10 (leaves collapse and turn yellow), with an increment of 1.; 20 degC, 12 hrs daylight |
|
| At1 CFU2 |
In planta bacterial growth (number of CFU / leaf area) of the P. viridiflava strain was individually measured as described in Goss and Bergelson 2006; 20 degC, 12 hrs daylight |
|
| As |
Four days after inoculation, leaves were scored by eye for disease symptom using a scale from 0 (no visible symptom) to 10 (leaves collapse and turn yellow), with an increment of 1.; 20 degC, 12 hrs daylight |
|
| As CFU2 |
In planta bacterial growth (number of CFU / leaf area) of the P. viridiflava strain was individually measured as described in Goss and Bergelson 2006; 20 degC, 12 hrs daylight |
|
| Bs |
Four days after inoculation, leaves were scored by eye for disease symptom using a scale from 0 (no visible symptom) to 10 (leaves collapse and turn yellow), with an increment of 1.; 20 degC, 12 hrs daylight |
|
| Bs CFU2 |
In planta bacterial growth (number of CFU / leaf area) of the P. viridiflava strain was individually measured as described in Goss and Bergelson 2006; 20 degC, 12 hrs daylight |
|
| At2 |
Four days after inoculation, leaves were scored by eye for disease symptom using a scale from 0 (no visible symptom) to 10 (leaves collapse and turn yellow), with an increment of 1.; 20 degC, 12 hrs daylight |
|
| At2 CFU2 |
In planta bacterial growth (number of CFU / leaf area) of the P. viridiflava strain was individually measured as described in Goss and Bergelson 2006; 20 degC, 12 hrs daylight |
|
| As2 |
Four days after inoculation, leaves were scored by eye for disease symptom using a scale from 0 (no visible symptom) to 10 (leaves collapse and turn yellow), with an increment of 1.; 20 degC, 12 hrs daylight |
|
| As2 CFU2 |
In planta bacterial growth (number of CFU / leaf area) of the P. viridiflava strain was individually measured as described in Goss and Bergelson 2006; 20 degC, 12 hrs daylight |
|
| FW |
Lesioning measured by fresh weight; 23 degC, 8hrs daylight (Short Day) for 7 weeks |
|
| DW |
Lesioning measured by dry weight; 23 degC, 8hrs daylight (Short Day) for 7 weeks |
|
| LES |
Lesioning is the presence in older leaves of necrotic spots, that spread to the whole leaf. Lesioned accessions were divided in three subgroups (mild, intermediate and severe) according to the severity of lesioning; 23 degC, 8hrs daylight (Short Day) for 7 weeks |
|
| YEL |
Yellowing is a precocious,more diffused leaf chlorosis.Accessions were divided in three subgroups (mild, intermediate and severe) according to the severity of the yellowing.; 23 degC, 8hrs daylight (Short Day) for 7 weeks |
|
| LY |
Presence of lesioning and yellowing.; 23 degC, 8hrs daylight (Short Day) for 7 weeks |
|
| LN10 |
Plants were checked bi-weekly for presence of first buds, and the average leaf number at flowering time of 4 plants of the same accession were collected; 10 degC, 16 hrs daylight |
|
| LN16 |
Plants were checked bi-weekly for presence of first buds, and the average leaf number at flowering time of 4 plants of the same accession were collected; 16 degC, 16 hrs daylight |
|
| LN22 |
Plants were checked bi-weekly for presence of first buds, and the average leaf number at flowering time of 4 plants of the same accession were collected; 22 degC, 16 hrs daylight |
|
| Silique 16 |
The length of 5 siliques was measured for each accession after growth had concluded; 16 degC, 16 hrs daylight |
|
| Silique 22 |
Lesioning measured by fresh weight; 22 degC, 16 hrs daylight |
|
| Germ 10 |
Days to germination of each accession were recorded daily at each temperature upon first emergence of cotyledons; 10 degC, 16 hrs daylight |
|
| Germ 16 |
Days to germination of each accession were recorded daily at each temperature upon first emergence of cotyledons; 16 degC, 16 hrs daylight |
|
| Germ 22 |
Days to germination of each accession were recorded daily at each temperature upon first emergence of cotyledons; 22 degC, 16 hrs daylight |
|
| Width 10 |
The diameters of 4 plants of each accession were measured and the results were expressed as an average value across all available replicates.Plants were scored 8 weeks post germination; 10 degC, 16 hrs daylight |
|
| Width 16 |
The diameters of 4 plants of each accession were measured and the results were expressed as an average value across all available replicates.Plants were scored 5 weeks post germination; 16 degC, 16 hrs daylight |
|
| Width 22 |
The diameters of 4 plants of each accession were measured and the results were expressed as an average value across all available replicates.Plants were scored 5 weeks post germination; 22 degC, 16 hrs daylight |
|
| Chlorosis 10 |
Results expressed as binary data, determined by the presence (1) or absence (0) of chlorosis in all 4 plants / accession after 8wks of growth; 10 degC, 16 hrs daylight |
|
| Chlorosis 16 |
Results expressed as binary data, determined by the presence (1) or absence (0) of chlorosis in all 4 plants / accession after 5wks of growth; 16 degC, 16 hrs daylight |
|
| Chlorosis 22 |
Results expressed as binary data, determined by the presence (1) or absence (0) of chlorosis in all 4 plants / accession after 5wks of growth; 22 degC, 16 hrs daylight |
|
| Anthocyanin 10 |
Results expressed as binary data, determined by the presence (1) or absence (0) of anthocyanin in all 4 plants / accession after 8wks of growth; 10 degC, 16 hrs daylight |
|
| Anthocyanin 16 |
Results expressed as binary data, determined by the presence (1) or absence (0) of anthocyanin in all 4 plants / accession after 5wks of growth; 16 degC, 16 hrs daylight |
|
| Anthocyanin 22 |
Results expressed as binary data, determined by the presence (1) or absence (0) of anthocyanin in all 4 plants / accession after 5wks of growth; 22 degC, 16 hrs daylight |
|
| Leaf serr 10 |
Average severity of serration across 4 plants was scored between 0 (entire lamina) and 1.5 (jagged serration) after 8 weeks of growth; 10 degC, 16 hrs daylight |
|
| Leaf serr 16 |
Average severity of serration across 4 plants was scored between 0 (entire lamina) and 1.5 (jagged serration) after 5 weeks of growth; 16 degC, 16 hrs daylight |
|
| Leaf serr 22 |
Average severity of serration across 4 plants was scored between 0 (entire lamina) and 1.5 (jagged serration) after 5 weeks of growth; 22 degC, 16 hrs daylight |
|
| Leaf roll 10 |
Results expressed as binary data, determined by the presence (1) or absence (0) of rolled leaves in all 4 plants / accession at 8 weeks growth; 10 degC, 16 hrs daylight |
|
| Leaf roll 16 |
Results expressed as binary data, determined by the presence (1) or absence (0) of rolled leaves in all 4 plants / accession at 5 weeks growth; 16 degC, 16 hrs daylight |
|
| Leaf roll 22 |
Results expressed as binary data, determined by the presence (1) or absence (0) of rolled leaves in all 4 plants / accession at 5 weeks growth; 22 degC, 16 hrs daylight |
|
| Rosette Erect 22 |
(22 degC was the only temperature at which this phenotype was frequently observed). Results were expressed as binary data, determined by the central rosette being erect (1) or not (0) in all 4 plants / accession phenotyped at 5 weeks post germination; 22 degC, 16 hrs daylight |
|
| Hypocotyl length |
After seven days growth under the photocycle and thermocycle treatment,plants were flattened directly on the agar and imaged on a flatbed scanner. Hypocotyl lengths were determined using NIH Image; Seeds were vapour sterilized, plated in a random design on 1/2 MS agar plates (-sucrose), stratified for 4 days at 4 degC in the dark, before transferring to photocycles (12 h 100 μ E/m cool white light / 12 hours dark |
|
| Trichome avg C |
Measurements were collected from leaf disks removed from the 11th leaf of plants that had been treated with 0.6ml of a water control (C). Trichome density was calculated as the trichome number per disk divided by the disk area; 20 degC, 12 hrs daylight |
|
| Trichome avg JA |
Measurements were collected from leaf disks removed from the 11th leaf of plants that had been treated with 0.6 ml of a 0.45mM solution of jasmonic acid (Sigma J-2500) in water (JA). Trichome density was calculated as the trichome number per disk divided by the disk area; 20 degC, 12 hrs daylight |
|
| Aphid number |
On day 25 of growth,two alate females of the common peach aphid, Myzus persicae,were placed on each of four plants of each of the 96 genotypes. Nine days later, the number of offspring produced by these aphids on each plant was recorded.; 20 degC, 12 hrs daylight |
|
| Bacterial titer |
Following inoculation into leaf tissues, the titers of bacteria were measured at 0 and 4 days. Bacterial titres were measured from hole-punched leaf disks ground in 200μL of 10 mM MgSO4. Measurements were expressed as colony forming units per unit area and replicated in triplicate.; 20 degC, 12 hrs daylight |
|
| Seedling Growth |
Seedling growth rate was given by leaf area divided by the number of days of growth since sowing ( in cm2/day); Seeds were grown for one week in the greenhouse under long day (16 hours light) |
|
| Vern Growth |
Vegetative growth rate during vernalization was estimated as the increment of cm2 leaf area per day between each of the three time points for which leaf area was measured; Seeds were grown for one week in the greenhouse under long day (16 hours light), vernalized for 4 weeks (4 degC, 16h light, 50% relative humidity) |
|
| After Vern Growth |
Vegetative growth rate after vernalization was estimated as the increment of cm2 leaf area per day between each of the three time points for which leaf area was measured; Seeds were grown for one week in the greenhouse under long day (16 hours light), vernalized for 4 weeks (4 degC, 16h light, 50% relative humidity)and then returned to greenhouse |
|
| Secondary Dormancy |
Secondary dormancy was given by the slope between the germination percentages of non-dormant seeds after one and six weeks of cold treatment. Viability of non germinating seeds after cold treatment was confirmed as described in Cadman et al., 2006; Fully after-ripened seeds were treated with a 1- and 6-week long exposure to 4 degC |
|
| Germ in dark |
The ability to germinate in the dark at 4 degC was measured as the percentage of non dormant seeds that can germinate during 1-week long cold exposure, in the absence of light; 4 degC, in the dark |
|
| DSDS50 |
Number of days of seed dry storage required to reach 50% germination, or DSDS50 value (Alonso-Blanco et al., 2003); Dry storage, followed by 25 degC, 12h day, 20 degC, 12h night for 1 week |
|
| Seed bank 133-91 |
Genotypes consistently decreased their percentage of germination between 91 and 133 days of dry storage. The time point for this sudden reduction in germination rate was scored by the slope between germination percentages at these two time points; Between 91 and 133 days of dry storage |
|
| Storage 7 days |
Primary dormancy was measured as the progressive increase of germination rate measured after 7 days of dry storage; 7 days dry storage as described in Alonso-Blanco et al, 2003 |
|
| Storage 28 days |
Primary dormancy was measured as the progressive increase of germination rate measured after 28 days of dry storage; 28 days dry storage as described in Alonso-Blanco et al, 2003 |
|
| Storage 56 days |
Primary dormancy was measured as the progressive increase of germination rate measured after 56 days of dry storage; 56 days dry storage as described in Alonso-Blanco et al, 2003 |
|
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Arabidopsis thaliana Germplasm "Ren-1"
Experiments (1)
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