Title | Rapid survey for presence of a blast resistance gene Pi-ta in rice cultivars using the dominant DNA markers derived from portions of the Pi-ta gene |
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Design | 141 accessions of rice, Oryza sativa, collected from major rice-producing regions throughout the world, 'Katy', 'Drew', and 'Kaybonnet' (resistant to M. grisea) and Nipponbare and a California cultivar 'M-202' (susceptible to M. grisea) were used as positive and negative control respectively in the pathogenicity assay. M. grisea race IC-17 containing AVR-Pita was used in the assay. |
Polymorphism Type (0) | None |
Allele Scoring Protocol (0) | |
PI(s) | Y. Jia |
Comments | Reference: Wang Z, et. al. [Gramene Lit ID 54913] |
Trait Name | Trait Ontology Accession | Phenotype Measurement |
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blast disease | TO:0000074 | PATO:0001178; resistant to; ECO:0000002; direct assay result; EO:0007226; Magnaporthe grisea; "Phenotypes (resistance or susceptibility) were scored 7 days after inoculation. Resistance was scored based on the absence of visible infection and conidia in inoculated tissue, while susceptibility was scored by the presence of lesion (size >3 mm in length) and the presence of conidia in the lesions." |