Plant Breeding Institute, Faculty of Agriculture, Hokkaido University, Sapporo, 060 Japan
Anthers inoculated on the medium and anther-derived calli were
irradiated with acute and chronic gamma-rays with four kinds of doses. It
was noted that induction of somaclonal mutants remarkably increased by
gamma-irradiation with the dose of 20 kR or 30 kR in chronic gamma-rays
(Table 1). Though the
Table 1. Effect of gamma-ray irradiation on anther and anther callus
============================================================================== Treatment No. of No. of M\5\ Lines Frequency (%) of mutant lines M\2\ ====================== ================================ lines Normal mutant Total M\2\ M\3\ M\5\ (fix.) (fix.) mutants mutants mutants /M\2\ /M\2\ /M\2\ ============================================================================== Anther 0.5 kR 57 0 6 6 36.8 5.3 10.5 1.0 kR 9 0 2 2 55.6 22.2 22.2 20 kR 31 0 9 9 61.3 16.1 29.0 30 kR 13 0 3 3 61.5 38.5 23.1 Anther callus 0.5 kR 51 1 1 2 29.4 3.9 2.0 1.0 kR 21 0 3 3 33.3 14.3 14.3 20 kR 23 0 10 10 78.3 43.5 43.5 30 kR 30 1 10 11 80.0 50.0 33.3 Anther culture non-irradiation 36 0 3 3 38.9 8.3 8.3 Control 23 1 0 1 0.0 0.0 0.0 ============================================================================== *Doubled haploid lines produced from diploid plants regenerated from anther calli (M\1\R\1\ or the 1st generation of mutagenesis and regeneration). M\2\ - 2nd generation of mutagenesis and regeneration.
================================================================================ Character1) ========================================================== Strain Treat. CL PL PN SL sw NS SF HP (cm) (cm) (mm) (mm) (%) (days) ================================================================================ Kitaake Control 52.0 14.7 12.1 5.89 3.59 59.6 94.3 87.7 (100) (100) (100) (100) (100) (100) (100) (100) AT-49 30 kR 36.9** 13.3* 23.2** 5.79 3.28** 17.6** 75.4* 86.6 (Anther) (68) (91) (192) (98) (91) (30) (80) (99) AT-52 30 kR 51.3 14.3 10.6 5.97 3.67* 51.8 89.2* 91.5 (Anther) (98) (97) (88) (101) (102) (87) (95) (104) AT-127 1.0 kR 48.3 9.9** 13.3 5.37** 3.43 17.8** 87.6 91.8 (Anther) (93) (67) (110) (91) (96) (30) (93) (105) AT-130 20 kR 50.0 13.7* 16.6* 4.98** 2.87** 77.3* 39.8**112.0* (Callus) (96) (93) (137) (85) (80) (130) (42) (128) AT-173 30 kR 32.7** 9.3* 5.8* 5.38* 3.48 21.7** 1.2**92.3 (Callus) (63) (67) (47) (91) (97) (36) (1) (105) AT-307 (non-irrad.) 48.1 13.0* 12.6 5.88 3.62* 49.3 89.5 87.8 (92) (88) (104) (100) (101) (83) (95) (100) ================================================================================ 1) CL: Culm length, PL: Panicle length, PN: Panicle number, SL: Spikelet length, SW: Spikelet width, NS: Number of spikelets per panicle, SF: Seed fertility, HP: Heading period. *, **; Significantly different from Kitaake at the 5% and 1% levels, respectively.
============================================================================= Cross Mutant F\2\ segregation Goodness of fit combination character ================ Total ===================== Normal mutant X2(3:1) p ============================================================================= AT-49xNormal dwarf 177 56 233 0.12 0.8-0.7 AT-52xNormal brown leaf spot 268 97 365 0.48 0.5-0.4 AT-127xNormal short panicle 575 188 763 0.05 0.9-0.8 AT-13OxNormal small grain 435 117 552 4.26 .05-.01 AT-173xNormal brittle culm 103 30 133 0.42 0.6-0.5 AT-307xNormal reduced panicle 831 250 1081 2.02 0.2-0.1 =============================================================================
Agronomic characters in six mutant lines (AT-lines) which were selected on the basis of altered phenotype indicated that these lines were different from the original variety, 'Kitaake'. Seed sterility, dwarfness and grain size were frequently altered in AT-lines. It seems that most of the mutant genes exhibit pleiotropic effects on several characters. Genic analyses indicated that most of the mutant characters were due to a single recessive gene. Dwarf mutant with short panicle in AT-49, small brown leaf spots on leaf blades in AT-52, short panicle in AT-127, small grain in AT-130, brittle culm in AT-173 and ruduced spikelets on the panicle tip in AT-307 were governed by single recessive genes, respectively (Table 2, 3).
References
Novak, F.J., S. Daskalov, H. Brunner, M. Nesticky, R. Afza, M. Dolezelova, S. Lucretti, A. Herichova and T. Hermelin, 1988. Somatic embryogenesis in maize and comparison of genetic variability induced by gamma radiation and tissue culture techniques. Plant Breed. 101: 66-79.