Marker | Chr. | Position cM | TIGRv3 | Forward Primer (Labeled) | Reverse Primer | Anneal temp | PCR Cycles | Panel | Label | Color | Pooling conc. | Min Allele | Max Allele |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
RM495* | 1 | 2.8 | 213775 | aatccaaggtgcagagatgg | caacgatgacgaacacaacc | 55 | 30 | 8 | 6FAM | blue | 1x | 148 | 160 |
RM1 | 1 | 29.7 | 4633595 | gcgaaaacacaatgcaaaaa | gcgttggttggacctgac | 55 | 30 | 2 | PET | red | 1x | 67 | 119 |
RM283* | 1 | 31.4 | 4883717 | gtctacatgtacccttgttggg | cggcatgagagtctgtgatg | 61 | 30 | 4 | NED | yellow | 1x | 130 | 176 |
RM259 | 1 | 54.2 | 7443424 | tggagtttgagaggaggg | cttgttgcatggtgccatgt | 55 | 30 | 1 | 6FAM | blue | 2x | 133 | 186 |
RM312 | 1 | 71.6 | 14890771 | gtatgcatatttgataagag | aagtcaccgagtttaccttc | 55 | 30 | 1 | 6FAM | blue | 1x | 86 | 106 |
RM5 | 1 | 94.9 | 23952076 | tgcaacttctagctgctcga | gcatccgatcttgatggg | 57 | 30 | 5 | VIC | green | 1x | 94 | 138 |
RM237* | 1 | 115.2 | 26795365 | caaatcccgactgctgtcc | tgggaagagagcactacagc | 55 | 30 | 6 | VIC | green | 1x | 105 | 153 |
RM431* | 1 | 178.3 | 38874702 | tcctgcgaactgaagagttg | agagcaaaaccctggttcac | 55 | 30 | 2 | VIC | green | 1x | 233 | 261 |
RM154* | 2 | 4.8 | 1083895 | accctctccgcctcgcctcctc | ctcctcctcctgcgaccgctcc | 61 | 30 | 9 | PET | red | 1x | 148 | 230 |
RM452* | 2 | 58.4 | 9564377 | ctgatcgagagcgttaaggg | gggatcaaaccacgtttctg | 61 | 30 | 1 | 6FAM | blue | 1x | 192 | 213 |
RM489 | 3 | 29.2 | 4316348 | acttgagacgatcggacacc | tcacccatggatgttgtcag | 55 | 30 | 4 | PET | red | 2x | 223 | 289 |
OSR13* | 3 | 53.1 | 7109988 | catttgtgcgtcacggagta | agccacagcgcccatctctc | 53 | 40 | 3 | VIC | green | 1x | 85 | 122 |
RM338* | 3 | 108.4 | 13210600 | cacaggagcaggagaagagc | ggcaaaccgatcactcagtc | 55 | 40 | 1 | VIC | green | 1x | 178 | 184 |
RM55 | 3 | 168.2 | 29002484 | ccgtcgccgtagtagagaag | tcccggttattttaaggcg | 55 | 30 | 1 | NED | yellow | 1x | 216 | 247 |
RM514* | 3 | 216.4 | 35229618 | agattgatctcccattcccc | cacgagcatattactagtgg | 55 | 30 | 4 | VIC | green | 1x | 229 | 278 |
RM307 | 4 | 0 | 12979675 | gtactaccgacctaccgttcac | ctgctatgcatgaactgctc | 55 | 30 | 3 | NED | yellow | 1x | 116 | 191 |
RM124* | 4 | 150.1 | 34485157 | atcgtctgcgttgcggctgctg | catggatcaccgagctcccccc | 67 | 30 | 2 | 6FAM | blue | 1x | 257 | 289 |
RM507* | 5 | 0 | 71397 | cttaagctccagccgaaatg | ctcaccctcatcatcgcc | 55 | 30 | 2 | NED | yellow | 1x | 234 | 257 |
RM413* | 5 | 26.7 | 2181391 | ggcgattcttggatgaagag | tccccaccaatcttgtcttc | 53 | 30 | 1 | PET | red | 1x | 71 | 114 |
RM161* | 5 | 96.9 | 20714463 | tgcagatgagaagcggcgcctc | tgtgtcatcagacggcgctccg | 61 | 30 | 9 | 6FAM | blue | 1x | 154 | 187 |
RM178 | 5 | 118.8 | 24923084 | tcgcgtgaaagataagcggcgc | gatcaccgttccctccgcctgc | 69 | 30 | 1 | NED | yellow | 1x | 112 | 131 |
RM334 | 5 | 141.8 | 28285978 | gttcagtgttcagtgccacc | gactttgatctttggtggacg | 55 | 30 | 9 | VIC | green | 2x | 119 | 207 |
RM133* | 6 | 0 | 226944 | ttggattgttttgctggctcgc | ggaacacggggtcggaagcgac | 63 | 30 | 9 | NED | yellow | 1x | 226 | 237 |
RM510 | 6 | 20.8 | 2831513 | aaccggattagtttctcgcc | tgaggacgacgagcagattc | 57 | 30 | 4 | VIC | green | 1x | 99 | 127 |
RM454 | 6 | 99.3 | 23336824 | ctcaagcttagctgctgctg | gtgatcagtgcaccatagcg | 55 | 30 | 3 | NED | yellow | 1x | 249 | 292 |
RM162* | 6 | 108.3 | 23991705 | gccagcaaaaccagggatccgg | caaggtcttgtgcggcttgcgg | 61 | 30 | 3 | PET | red | 1x | 191 | 244 |
RM125* | 7 | 24.8 | 5478776 | atcagcagccatggcagcgacc | aggggatcatgtgccgaaggcc | 63 | 30 | 6 | 6FAM | blue | 1x | 105 | 147 |
RM11 | 7 | 47 | 19256213 | tctcctcttcccccgatc | atagcgggcgaggcttag | 55 | 30 | 6 | PET | red | 2x | 118 | 151 |
RM455* | 7 | 65.7 | 22349919 | aacaacccaccacctgtctc | agaaggaaaagggctcgatc | 57 | 30 | 7 | PET | red | 1x | 127 | 144 |
RM118* | 7 | 96.9 | 26635903 | ccaatcggagccaccggagagc | cacatcctccagcgacgccgag | 67 | 30 | 7 | 6FAM | blue | 1x | 149 | 165 |
RM408* | 8 | 0 | 119935 | caacgagctaacttccgtcc | actgctacttgggtagctgacc | 55 | 30 | 3 | 6FAM | blue | 1x | 112 | 128 |
RM152* | 8 | 9.4 | 677616 | gaaaccaccacacctcaccg | ccgtagaccttcttgaagtag | 53 | 40 | 8 | PET | red | 1x | 133 | 157 |
RM25 | 8 | 52.2 | 4372113 | ggaaagaatgatcttttcatgg | ctaccatcaaaaccaatgttc | 53 | 40 | 1 | PET | red | 2x | 121 | 159 |
RM44* | 8 | 60.9 | 11753077 | acgggcaatccgaacaacc | tcgggaaaacctaccctacc | 53 | 30 | 4 | 6FAM | blue | 2x | 82 | 132 |
RM284* | 8 | 83.7 | 21012223 | atctctgatactccatccatcc | cctgtacgttgatccgaagc | 55 | 30 | 6 | NED | yellow | 1x | 139 | 159 |
RM433* | 8 | 116 | 25691233 | tgcgctgaactaaacacagc | agacaaacctggccattcac | 53 | 40 | 7 | PET | red | 1x | 216 | 248 |
RM447* | 8 | 124.6 | 26416867 | cccttgtgctgtctcctctc | acgggcttcttctccttctc | 55 | 30 | 5 | PET | red | 2x | 95 | 146 |
RM316* | 9 | 1.8 | 1022645 | ctagttgggcatacgatggc | acgcttatatgttacgtcaac | 55 | 30 | 7 | VIC | green | 1x | 194 | 216 |
RM105 | 9 | 32.1 | 12496919 | gtcgtcgacccatcggagccac | tggtcgaggtggggatcgggtc | 63 | 30 | 5 | 6FAM | blue | 1x | 100 | 141 |
RM215* | 9 | 99.4 | 20837148 | caaaatggagcagcaagagc | tgagcacctccttctctgtag | 55 | 30 | 2 | VIC | green | 1x | 126 | 161 |
RM474 | 10 | 0 | 1798783 | aagatgtacgggtggcattc | tatgagctggtgagcaatgg | 55 | 30 | 7 | 6FAM | blue | 2x | 216 | 288 |
RM271* | 10 | 59.4 | 16202474 | tcagatctacaattccatcc | tcggtgagacctagagagcc | 55 | 30 | 2 | 6FAM | blue | 1x | 80 | 120 |
RM171 | 10 | 73 | 18614310 | aacgcgaggacacgtacttac | acgagatacgtacgcctttg | 55 | 40 | 3 | 6FAM | blue | 1x | 307 | 347 |
RM484* | 10 | 97.3 | 20630348 | tctccctcctcaccattgtc | tgctgccctctctctctctc | 55 | 30 | 5 | 6FAM | blue | 2x | 286 | 298 |
RM552 | 11 | 40.6 | 4836203 | cgcagttgtggatttcagtg | tgctcaacgtttgactgtcc | 55 | 30 | 6 | 6FAM | blue | 1x | 167 | 258 |
RM536* | 11 | 55.1 | 8963471 | tctctcctcttgtttggctc | acacaccaacacgaccacac | 55 | 30 | 6 | PET | red | 2x | 223 | 247 |
RM287 | 11 | 68.6 | 16733868 | ttccctgttaagagagaaatc | gtgtatttggtgaaagcaac | 55 | 30 | 1 | VIC | green | 1x | 82 | 118 |
RM144 | 11 | 123.2 | 28158704 | tgccctggcgcaaatttgatcc | gctagaggagatcagatggtagtgcatg | 57 | 30 | 4 | NED | yellow | 1x | 216 | 295 |
RM19 | 12 | 20.9 | 2432429 | caaaaacagagcagatgac | ctcaagatggacgccaaga | 55 | 30 | 5 | PET | red | 1x | 192 | 250 |
RM277* | 12 | 57.2 | 18286130 | cggtcaaatcatcacctgac | caaggcttgcaagggaag | 55 | 30 | 2 | VIC | green | 1x | 104 | 121 |