This reports the protocol used to align the RiceRufipogon_BACend_OR_CB_OMAP-20070815 features to indica-genome. Tue Aug 21 13:22:20 2007 Source of RiceRufipogon_BACend_OR_CB_OMAP-20070815 : from Gramene markers database, originally Downloaded from genbank nucleotide databse with keyword 'OR_CBa' Alignment procedure details --------------------------- 70982 RiceRufipogon_BACend_OR_CB_OMAP-20070815 are aligned to indica-genome using blat with blat parameters -minIdentity=50 -minScore=10 -maxGap=3 followed by PslReps with -minNearTopSize=75 -minAli=0.25. This was followed by a filtering procedure described below and applied in general to 'OMAPSpecies-Genomic' data sets. Initial summary # alignments : 101015 # unique Features these alignments represent: 70547 % of total features these alignments represent : 99.39 % The length of the matches are distributed as follows Hit_length # alignments -------- -------- 100 234 150 586 200 710 250 840 300 951 350 976 400 1541 450 2062 500 2667 550 4045 600 7286 650 12153 700 17651 750 19914 800 16188 10000 13211 gap distribution of the remaining features gaps # alignments -------- -------- 1000 93687 2000 531 3000 376 4000 271 5000 240 6000 125 7000 102 8000 106 9000 111 10000 95 20000 461 Frequency distribution of the remaining features # hits # features -------- -------- 1 60064 2 4666 3 1961 4 1189 5 853 6 514 8 630 9 168 10 135 20 313 30 26 40 12 50 12 100 4 % Identity distribution of the remaining features % Identity # alignemnts -------- -------- 10 0 20 0 30 0 40 0 50 2 60 16 70 42 80 134 90 1493 100 99328 Following is the final summary # alignments : 101015 # unique Features these alignments represent: 70547 % of total features these alignments represent : 99.39 %
Last modified: Thu Sep 13 15:01:03 2007