This reports the protocol used to align the RiceOfficinalis_BACend_OO__B_OMAP-20070815 features to indica-genome. Tue Aug 21 12:54:33 2007 Source of RiceOfficinalis_BACend_OO__B_OMAP-20070815 : from Gramene markers database, originally Downloaded from genbank nucleotide databse with keyword 'OO__Ba' Alignment procedure details --------------------------- 101091 RiceOfficinalis_BACend_OO__B_OMAP-20070815 are aligned to indica-genome using blat with blat parameters -minIdentity=50 -minScore=10 -maxGap=3 followed by PslReps with -minNearTopSize=75 -minAli=0.25. This was followed by a filtering procedure described below and applied in general to 'OMAPSpecies-Genomic' data sets. Initial summary # alignments : 118553 # unique Features these alignments represent: 92465 % of total features these alignments represent : 91.47 % The length of the matches are distributed as follows Hit_length # alignments -------- -------- 100 4921 150 5207 200 5832 250 6155 300 6460 350 6890 400 7473 450 7793 500 8466 550 9865 600 10871 650 11807 700 10830 750 8496 800 4765 10000 2722 gap distribution of the remaining features gaps # alignments -------- -------- 1000 103121 2000 976 3000 804 4000 383 5000 324 6000 275 7000 160 8000 110 9000 139 10000 141 20000 1155 Frequency distribution of the remaining features # hits # features -------- -------- 1 79135 2 8172 3 2409 4 990 5 658 6 405 8 405 9 106 10 65 20 117 30 2 40 1 50 0 100 0 % Identity distribution of the remaining features % Identity # alignemnts -------- -------- 10 0 20 0 30 17 40 66 50 236 60 660 70 3239 80 11051 90 46500 100 56784 Following is the final summary # alignments : 118553 # unique Features these alignments represent: 92465 % of total features these alignments represent : 91.47 %
Last modified: Thu Sep 13 15:01:03 2007