This reports the protocol used to align the RiceNivara_BACend_OR_BB_OMAP-20070815 features to indica-genome. Tue Aug 21 14:27:24 2007 Source of RiceNivara_BACend_OR_BB_OMAP-20070815 : from Gramene markers database, originally Downloaded from genbank nucleotide databse with keyword 'OR_BBa' Alignment procedure details --------------------------- 106124 RiceNivara_BACend_OR_BB_OMAP-20070815 are aligned to indica-genome using blat with blat parameters -minIdentity=50 -minScore=10 -maxGap=3 followed by PslReps with -minNearTopSize=75 -minAli=0.25. This was followed by a filtering procedure described below and applied in general to 'OMAPSpecies-Genomic' data sets. Initial summary # alignments : 153241 # unique Features these alignments represent: 104703 % of total features these alignments represent : 98.66 % The length of the matches are distributed as follows Hit_length # alignments -------- -------- 100 443 150 889 200 1384 250 1698 300 2291 350 2417 400 3787 450 5424 500 8059 550 10973 600 16399 650 21609 700 25790 750 23970 800 16503 10000 11605 gap distribution of the remaining features gaps # alignments -------- -------- 1000 142324 2000 836 3000 569 4000 329 5000 340 6000 215 7000 147 8000 162 9000 190 10000 95 20000 629 Frequency distribution of the remaining features # hits # features -------- -------- 1 87805 2 7419 3 3186 4 1893 5 1424 6 842 8 1049 9 330 10 202 20 485 30 47 40 7 50 9 100 5 % Identity distribution of the remaining features % Identity # alignemnts -------- -------- 10 0 20 0 30 0 40 1 50 6 60 16 70 70 80 239 90 2549 100 150360 Following is the final summary # alignments : 153241 # unique Features these alignments represent: 104703 % of total features these alignments represent : 98.66 %
Last modified: Thu Sep 13 15:01:03 2007