This reports the protocol used to align the RiceMinuta_BACend_OM__B_OMAP-20070815 features to indica-genome. Tue Aug 21 14:17:15 2007 Source of RiceMinuta_BACend_OM__B_OMAP-20070815 : from Gramene markers database, originally Downloaded from genbank nucleotide databse with keyword 'OM__Ba' Alignment procedure details --------------------------- 169460 RiceMinuta_BACend_OM__B_OMAP-20070815 are aligned to indica-genome using blat with blat parameters -minIdentity=50 -minScore=10 -maxGap=3 followed by PslReps with -minNearTopSize=75 -minAli=0.25. This was followed by a filtering procedure described below and applied in general to 'OMAPSpecies-Genomic' data sets. Initial summary # alignments : 189959 # unique Features these alignments represent: 150039 % of total features these alignments represent : 88.54 % The length of the matches are distributed as follows Hit_length # alignments -------- -------- 100 10623 150 11771 200 13794 250 14556 300 15615 350 16910 400 17391 450 17818 500 18107 550 17485 600 15301 650 10473 700 6117 750 2738 800 958 10000 302 gap distribution of the remaining features gaps # alignments -------- -------- 1000 167297 2000 1155 3000 873 4000 601 5000 441 6000 381 7000 254 8000 216 9000 199 10000 195 20000 1493 Frequency distribution of the remaining features # hits # features -------- -------- 1 128729 2 13535 3 3791 4 1505 5 946 6 581 8 533 9 148 10 100 20 165 30 3 40 3 50 0 100 0 % Identity distribution of the remaining features % Identity # alignemnts -------- -------- 10 0 20 0 30 8 40 95 50 309 60 968 70 4533 80 18601 90 71932 100 93513 Following is the final summary # alignments : 189959 # unique Features these alignments represent: 150039 % of total features these alignments represent : 88.54 %
Last modified: Thu Sep 13 15:01:03 2007