This reports the protocol used to align the RiceGranulata_BACend_OG_AB_OMAP-20070815 features to indica-genome. Tue Aug 21 13:52:46 2007 Source of RiceGranulata_BACend_OG_AB_OMAP-20070815 : from Gramene markers database, originally Downloaded from genbank nucleotide databse with keyword 'OG_ABa' Alignment procedure details --------------------------- 138171 RiceGranulata_BACend_OG_AB_OMAP-20070815 are aligned to indica-genome using blat with blat parameters -minIdentity=50 -minScore=10 -maxGap=3 followed by PslReps with -minNearTopSize=75 -minAli=0.25. This was followed by a filtering procedure described below and applied in general to 'OMAPSpecies-Genomic' data sets. Initial summary # alignments : 102039 # unique Features these alignments represent: 84170 % of total features these alignments represent : 60.92 % The length of the matches are distributed as follows Hit_length # alignments -------- -------- 100 20479 150 12127 200 8923 250 7737 300 7721 350 7680 400 6705 450 5949 500 5578 550 5036 600 4455 650 3822 700 2657 750 1769 800 870 10000 531 gap distribution of the remaining features gaps # alignments -------- -------- 1000 92379 2000 438 3000 203 4000 281 5000 211 6000 146 7000 85 8000 82 9000 64 10000 68 20000 473 Frequency distribution of the remaining features # hits # features -------- -------- 1 72637 2 8338 3 1830 4 641 5 363 6 156 8 124 9 31 10 10 20 36 30 1 40 2 50 1 100 0 % Identity distribution of the remaining features % Identity # alignemnts -------- -------- 10 0 20 4 30 41 40 202 50 1093 60 3447 70 9526 80 14848 90 32995 100 39883 Following is the final summary # alignments : 102039 # unique Features these alignments represent: 84170 % of total features these alignments represent : 60.92 %
Last modified: Thu Sep 13 15:01:03 2007