This reports the protocol used to align the RiceGlaberrima_BACend_OG_BB_OMAP-20070815 features to indica-genome. Tue Aug 21 13:15:35 2007 Source of RiceGlaberrima_BACend_OG_BB_OMAP-20070815 : from Gramene markers database, originally Downloaded from genbank nucleotide databse with keyword 'OG_BBa' Alignment procedure details --------------------------- 66821 RiceGlaberrima_BACend_OG_BB_OMAP-20070815 are aligned to indica-genome using blat with blat parameters -minIdentity=50 -minScore=10 -maxGap=3 followed by PslReps with -minNearTopSize=75 -minAli=0.25. This was followed by a filtering procedure described below and applied in general to 'OMAPSpecies-Genomic' data sets. Initial summary # alignments : 83854 # unique Features these alignments represent: 64998 % of total features these alignments represent : 97.27 % The length of the matches are distributed as follows Hit_length # alignments -------- -------- 100 886 150 2365 200 3089 250 3482 300 4032 350 4291 400 4861 450 5075 500 5868 550 6568 600 8439 650 10009 700 10346 750 7895 800 4393 10000 2255 gap distribution of the remaining features gaps # alignments -------- -------- 1000 78743 2000 443 3000 266 4000 199 5000 197 6000 107 7000 105 8000 65 9000 58 10000 43 20000 391 Frequency distribution of the remaining features # hits # features -------- -------- 1 56741 2 4549 3 1523 4 696 5 554 6 322 8 308 9 93 10 65 20 134 30 8 40 1 50 2 100 2 % Identity distribution of the remaining features % Identity # alignemnts -------- -------- 10 0 20 0 30 1 40 2 50 5 60 27 70 78 80 274 90 2894 100 80573 Following is the final summary # alignments : 83854 # unique Features these alignments represent: 64998 % of total features these alignments represent : 97.27 %
Last modified: Thu Sep 13 15:01:03 2007