This reports the protocol used to align the RiceCoarctata_BACend_OC__B_OMAP-20070815 features to indica-genome. Tue Aug 21 14:18:23 2007 Source of RiceCoarctata_BACend_OC__B_OMAP-20070815 : from Gramene markers database, originally Downloaded from genbank nucleotide databse with keyword 'OC__Ba' Alignment procedure details --------------------------- 195285 RiceCoarctata_BACend_OC__B_OMAP-20070815 are aligned to indica-genome using blat with blat parameters -minIdentity=50 -minScore=10 -maxGap=3 followed by PslReps with -minNearTopSize=75 -minAli=0.25. This was followed by a filtering procedure described below and applied in general to 'OMAPSpecies-Genomic' data sets. Initial summary # alignments : 134968 # unique Features these alignments represent: 122018 % of total features these alignments represent : 62.48 % The length of the matches are distributed as follows Hit_length # alignments -------- -------- 100 19268 150 9953 200 9848 250 9066 300 9603 350 9462 400 9641 450 9503 500 10120 550 9940 600 9116 650 7427 700 5470 750 3459 800 1897 10000 1195 gap distribution of the remaining features gaps # alignments -------- -------- 1000 129903 2000 1037 3000 352 4000 269 5000 342 6000 156 7000 114 8000 113 9000 49 10000 99 20000 527 Frequency distribution of the remaining features # hits # features -------- -------- 1 114272 2 5638 3 1111 4 338 5 371 6 156 8 48 9 10 10 12 20 39 30 17 40 2 50 1 100 3 % Identity distribution of the remaining features % Identity # alignemnts -------- -------- 10 0 20 2 30 35 40 134 50 580 60 2152 70 4242 80 8467 90 36621 100 82735 Following is the final summary # alignments : 134968 # unique Features these alignments represent: 122018 % of total features these alignments represent : 62.48 %
Last modified: Thu Sep 13 15:01:03 2007